Regulation of interleukin-4 signaling by extracellular reduction of intramolecular disulfides
2009 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 390, no 4, 1272-1277 p.Article in journal (Refereed) Published
Interleukin-4 (IL-4) contains three structurally important intramolecular disulfides that are required for the bioactivity of the cytokine. We show that the cell surface of HeLa cells and endotoxin-activated monocytes can reduce IL-4 intramolecular disulfides in the extracellular space and inhibit binding of IL-4 to the IL-4R alpha receptor. IL-4 disulfides were in vitro reduced by thioredoxin 1 (Trx1) and protein disulfide isomerase (PDI). Reduction of IL-4 disulfides by the cell surface of HeLa cells was inhibited by auranofin, an inhibitor of thioredoxin reductase that is an electron donor to both Trx1 and PDI. Both Trx1 and PDI have been shown to be located at the cell surface and our data suggests that these enzymes are involved in catalyzing reduction of IL-4 disulfides. The pro-drug N-acetylcysteine (NAC) that promotes T-helper type 1 responses was also shown to mediate the reduction of IL-4 disulfides. Our data provides evidence for a novel redox dependent pathway for regulation of cytokine activity by extracellular reduction of intramolecular disulfides at the cell surface by members of the thioredoxin enzyme family.
Place, publisher, year, edition, pages
2009. Vol. 390, no 4, 1272-1277 p.
Redox regulation, Cell surface redox reactions, Cytokines, Disulfides, Immune system regulation
IdentifiersURN: urn:nbn:se:su:diva-59407DOI: 10.1016/j.bbrc.2009.10.134ISI: 000272650800036OAI: oai:DiVA.org:su-59407DiVA: diva2:427443
authorCount :92011-06-282011-06-272011-06-28Bibliographically approved