Structural studies of the capsular polysaccharide produced by Leuconostoc mesenteroides ssp. cremoris PIA2
2011 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 12, no 7, 2496-2501 p.Article in journal (Refereed) Published
The structure of the capsular polysaccharide (CPS) produced by Leuconostoc mesenteroides ssp. cremoris PIA2 has been determined using component analysis and NMR spectroscopy. 1H and 13C resonances were assigned using 2D NMR experiments, and sequential information was obtained by 1H,1H-NOESY and 1H,13C-HMBC experiments. The CPS consists of linear pentasaccharide repeating units with the following structure: →3)-β-d-Galf-(1→6)-β-d-Galf-(1→2)-β-d-Galf-(1→6)-β-d-Galf-(1→3)-β-d-Galp-(1→, in which four out of the five sugar residues have the furanoid ring form, a structural entity found in bacteria but not in mammals. The analysis of the magnitude of the homonuclear three-bond coupling constants of the anomeric protons for the five-membered sugar rings indicates that the sugar residues substituted at a primary carbon atom show one kind of conformational preferences, whereas those substituted at a secondary carbon atom show another kind of conformational preferences.
Place, publisher, year, edition, pages
2011. Vol. 12, no 7, 2496-2501 p.
Research subject Organic Chemistry
IdentifiersURN: urn:nbn:se:su:diva-59717DOI: 10.1021/bm200177zISI: 000292617700009OAI: oai:DiVA.org:su-59717DiVA: diva2:429994
FunderSwedish Research CouncilKnut and Alice Wallenberg Foundation