Structural elucidation of the O-antigenic polysaccharide from Escherichia coli O175
2011 (English)In: Carbohydrate Research, ISSN 0008-6215, E-ISSN 1873-426X, Vol. 346, no 3, 449-453 p.Article in journal (Refereed) Published
The structure of the O-antigen polysaccharide (PS) from Escherichia coli O175 has been elucidated. Component analysis together with 1H and 13C NMR spectroscopy experiments were used to determine the structure. Inter-residue correlations were determined by 1H,1H-NOESY, and 1H,13C-heteronuclear multiple-bond correlation experiments. The PS is composed of pentasaccharide repeating units with the following structure:
Cross-peaks of low intensity from an α-linked glucopyranosyl residue were present in the 1H,1H-TOCSY NMR spectra. The α-d-Glcp residue is suggested to originate from the terminal part of the polysaccharide and consequently the biological repeating unit has a 3-substituted N-acetyl-d-galactosamine residue at its reducing end. The repeating unit of the E. coli O175 O-antigen is similar to those from E. coli O22 and O83, both of which carry an α-d-Glcp-(1→4)-d-GlcpA structural element, thereby explaining the reported cross-reactivities between the strains.
Place, publisher, year, edition, pages
2011. Vol. 346, no 3, 449-453 p.
Escherichia coli, lipopolysaccharide, NMR, biological repeating unit
Research subject Organic Chemistry
IdentifiersURN: urn:nbn:se:su:diva-59722DOI: 10.1016/j.carres.2010.12.005ISI: 000293037400010OAI: oai:DiVA.org:su-59722DiVA: diva2:430021
FunderSwedish Research CouncilKnut and Alice Wallenberg Foundation