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Development of a high performance anion exchange chromatography analysis for mapping of oligosaccharides
Stockholm University, Faculty of Science, Department of Analytical Chemistry.
2009 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 877, no 20-21, 1827-1832 p.Article in journal (Refereed) Published
Abstract [en]

In the present study a HPAEC-PAD method is described that was developed for monitoring the consistency of N-glycosylation during the production and purification of recombinant proteins and monoclonal antibodies. The method successfully separated 18 neutral and sialylated oligosaccharides. Results obtained were compared with MALDI-TOF MS and it was shown that both methods gave similar results. in addition, a method validation was performed showing that the HPAEC-PAD analysis was well suited for the mapping and characterization of oligosaccharides. The method was found to be robust and additionally the precision was significantly better compared to the MALDI-TOF MS method. 

Place, publisher, year, edition, pages
2009. Vol. 877, no 20-21, 1827-1832 p.
Keyword [en]
HPAEC-PAD, MALDI-TOF MS, Oligosaccharides, N-glycosylation, Glycoprotein, Monoclonal antibodies
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:su:diva-59966DOI: 10.1016/j.jchromb.2009.05.003ISI: 000267453800006OAI: oai:DiVA.org:su-59966DiVA: diva2:432527
Note
authorCount :4Available from: 2011-08-04 Created: 2011-08-02 Last updated: 2017-12-08Bibliographically approved

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