Change search
ReferencesLink to record
Permanent link

Direct link
Stockholm University, Faculty of Science, The Wenner-Gren Institute .
2009 (English)In: Methods in Enzymology, ISSN 0076-6879, Vol. 457, 211-229 p.Article, review/survey (Refereed) Published
Abstract [en]

Genome sequencing projects have provided the substrate for an unimaginable number of biological experiments. Further, genomic technologies such as microarrays and quantitative and exquisitely sensitive techniques such as real-time quantitative polymerase chain reaction have made it possible to reliably generate millions of data points per experiment. The data can be high quality and yield entirely new insights into how gene expression is coordinated under complex physiological situations. It can also be that the data and interpretation are meaningless because of a lack of physiological context or experimental control. Thus, functional genomics is now being applied to study metabolic physiology with varying degrees of success. From the genome sequencing projects we also have the information needed to design chemical tools that can knock down a gene transcript, even distinguishing between splice variants in mammalian cells. Use of such technologies, inspired by nature's endogenous RNAi mechanism-microRNA targeting, comes with significant caveats. While the discipline of Pharmacology taught us last century that inhibitor action specificity is dependent on the concentration used, these experiences have been ignored by users of siRNA technologies. What we provide in this chapter is some considerations and observations from functional genomic studies. We are largely concerned with the phase that follows a microarray study, where a candidate gene is selected for manipulation in a system that is considered to be simpler than the in vivo mammalian tissue and thus the methods discussed largely apply to this cell biology phase. We apologize for not referring to all relevant publications and for any technical considerations we have also failed to factor into our discussion.

Place, publisher, year, edition, pages
2009. Vol. 457, 211-229 p.
Keyword [en]
human skeletal-muscle, diploid cell strains, mitochondrial-function, gene-expression, dopaminergic-neurons, antisense rna, insulin, exercise, humans, sirna
National Category
Natural Sciences
URN: urn:nbn:se:su:diva-60059DOI: 10.1016/S0076-6879(09)05012-5ISI: 000266544100012OAI: diva2:433739
authorCount :3Available from: 2011-08-11 Created: 2011-08-08 Last updated: 2011-08-11Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text
By organisation
The Wenner-Gren Institute
In the same journal
Methods in Enzymology
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 18 hits
ReferencesLink to record
Permanent link

Direct link