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Genomics, transcriptomics and peptidomics of Daphnia pulex neuropeptides and protein hormones
Stockholm University, Faculty of Science, Department of Zoology, Functional Morphology.ORCID iD: 0000-0001-7815-4868
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2011 (English)In: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 10, no 10, 4478-4504 p.Article in journal (Refereed) Published
Abstract [en]

We report 43 novel genes in the water flea Daphnia pulex encoding 73 predicted neuropeptide and protein hormones as partly confirmed by RT-PCR. MALDI-TOF mass spectrometry identified 40 neuropeptides by mass matches and 30 neuropeptides by fragmentation sequencing. Single genes encode adipokinetic hormone, allatostatin-A, allatostatin-B, a first crustacean allatotropin, Ala7-CCAP, one CCHamide, Arg7-corazonin, CRF-like (DH52) and calcitonin-like (DH31) diuretic hormones, two ecdysis-triggering hormones, two FIRFamides, one insulin- and one each of three IGF-related peptides, two alternative splice forms of short and long ion transport peptide (ITP), one each of two N-terminally elongated ITPs, myosuppressin, neuroparsin, two neuropeptide-F splice forms, three periviscerokinins (but no pyrokinins), pigment dispersing hormone, proctolin, Met4-proctolin, one novel short neuropeptide-F, three RYamides, SIFamide, two sulfakinins, three tachykinins. Two genes encode orcokinins, three genes different allatostatins-C. Paired gene clusters occur for two novel eclosion hormones; bursicons alpha, beta; glycoproteins GPA2, GPB5; and two of the allatostatin-C genes. Detailed comparisons of genes or their products with those from insects and decapod crustaceans revealed that the D. pulex peptides are often closer to their insect than to their decapod crustacean homologues, confirming that branchiopods, to which Daphnia belongs, are the ancestor group of insects.

Place, publisher, year, edition, pages
2011. Vol. 10, no 10, 4478-4504 p.
Keyword [en]
Daphnia pulex, crustacean, neuropeptide, protein hormone, preprohormone, genomics, transcriptomics, bioinformatics, MALDI TOF mass spectrometry, RTPCR
National Category
Biochemistry and Molecular Biology Zoology
Research subject
URN: urn:nbn:se:su:diva-60282DOI: 10.1021/pr200284eISI: 000295602700011OAI: diva2:433947
Carl Tryggers Stiftelse
Available from: 2011-08-18 Created: 2011-08-11 Last updated: 2014-10-13Bibliographically approved

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Dircksen, HeinrichStrauss, Johannes
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