The T cell receptor resides in small ordered plasma membrane domains that aggregate upon T cell activation
(English)Manuscript (preprint) (Other academic)
T cell signaling emanates from large lipid raft platforms. Whether lipid rafts form upon T cell receptor (TCR) engagement or exist in resting T cells was the focus of this study. Plasma membrane order was followed in live T cells at 37°C using laurdan to report on lipid packing. Patching of the TCR in both Jurkat and human primary CD4+ T cells resulted in higher fractions of ordered plasma domains in the patches but did not increase the overall membrane order. The TCR colocalized with actin filaments in unstimulated Jurkat T cells and this colocalization was most prominent for cells in G1 phase. Moreover, the TCR located to the nuclear envelope, in addition to the plasma membrane, in cells in S and G2/M phase. Our study suggests that the TCR resides in ordered plasma membrane domains/lipid rafts that are linked to actin filament and aggregate upon T cell activation.
actin, generalised polarisation, lipid rafts, membrane order, T cell receptor
Research subject Cellbiology
IdentifiersURN: urn:nbn:se:su:diva-62288OAI: oai:DiVA.org:su-62288DiVA: diva2:441172