Structural studies of the O-antigenic polysaccharide from Escherichia coli O177
2011 (English)In: Carbohydrate Research, ISSN 0008-6215, E-ISSN 1873-426X, Vol. 346, no 14, 2300-2303 p.Article in journal (Refereed) Published
The structure of the O-antigen polysaccharide (PS) from Escherichia coli O177 has been determined. Component analysis together with 1H and 13C NMR spectroscopy experiments was used to determine the structure. Inter-residue correlations were determined by 1H,13C-heteronuclear multiple-bond correlation and 1H,1H-NOESY experiments. PS is composed of tetrasaccharide repeating units with the following structure:
An α-l-Rhap residue is suggested to be present at the terminal part of the polysaccharide, which on average is composed of ∼20 repeating units, since the 1H and 13C chemical shifts of an α-linked rhamnopyranosyl group could be assigned by a combination of 2D NMR spectra. Consequently, the biological repeating unit has a 3-substituted N-acetyl-d-glucosamine residue at its reducing end. The repeating unit of the E. coli O177 O-antigen shares the →3)-α-l-FucpNAc-(1→3)-β-d-GlcpNAc-(1→ structural element with the O-antigen from E. coli O15 and this identity may then explain the reported cross-reactivity between the strains.
Place, publisher, year, edition, pages
2011. Vol. 346, no 14, 2300-2303 p.
Escherichia coli, Lipopolysaccharide, NMR, Biological repeating unit, Cross-reactivity
Research subject Organic Chemistry
IdentifiersURN: urn:nbn:se:su:diva-62339DOI: 10.1016/j.carres.2011.06.022ISI: 000295754100034OAI: oai:DiVA.org:su-62339DiVA: diva2:441298
FunderSwedish Research CouncilKnut and Alice Wallenberg Foundation