The cell walls of bacteria are composed to a large extent of different polysaccharides. In order to understand and thereby, be able to utilise the physical, biological and immunological properties of polysaccharides, it is important to determine their structures. In the present thesis, structural investigations of the O-antigen polysaccharides from five strains of Escherichia coli, serogroups O138, O164, O167, O173 and O159, are described.
Nuclear Magnetic Resonance (NMR) spectroscopy was the primary technique employed. Fast Atom Bombardment Mass Spectrometry (FAB-MS) and Electrospray Mass Spectrometry (ESI-MS) together with chemical degradations of the polysaccharides were also utilised.
The possibilities, advantages and disadvantages of certain available methods for such studies are discussed.
Stockholm: Stockholm University , 1999. , 46 p.