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Learning in a simple biological system: A pilot study of classical conditioning of human macrophages in vitro
Stockholm University, Faculty of Social Sciences, Stress Research Institute. (Enheten för psykoneuroimmunologi)
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2011 (English)In: Behavioral and Brain Functions, ISSN 1744-9081, Vol. 7, 47- p.Article in journal (Refereed) Published
Abstract [en]

ABSTRACT: Recent advances in cell biology and gene regulation suggest mechanisms whereby associative learning could be performed by single cells. Therefore, we explored a model of classical conditioning in human macrophages in vitro. In macrophage cultures, bacterial lipopolysaccharide (LPS; unconditioned stimulus) was paired once with streptomycin (conditioned stimulus). Secretion of interleukin-6 (IL-6) was used as response measure. At evocation, conditioning was not observed. Levels of IL-6 were higher only in those cultures that had been exposed to LPS in the learning phase (p's<.05), regardless whether they received the conditioned stimulus or not at evocation. However, habituation was evident, with a 62% loss of the IL-6 response after three LPS presentations (p<.001). If further experiments confirm that simple learning can occur in immune cells, this may have bearings not only on immune regulation, but also on the brain response to molecular signals detected in the periphery. Importantly, whether capacities for simple learning in single cells extend beyond habituation, and how this would be demonstrated, remain open questions.

Place, publisher, year, edition, pages
2011. Vol. 7, 47- p.
Keyword [en]
associative learning; conditioning; habituation; in vitro; monocyte; macrophage; lipopolysaccharide; streptomycin; interleukin-6
National Category
Medical and Health Sciences
URN: urn:nbn:se:su:diva-64793DOI: 10.1186/1744-9081-7-47ISI: 000298364700001PubMedID: 22098673Local ID: P2888OAI: diva2:458755
Available from: 2011-11-23 Created: 2011-11-23 Last updated: 2012-01-29Bibliographically approved

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Nilsonne, GustavLekander, Mats
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