Change search
ReferencesLink to record
Permanent link

Direct link
A reference map of the membrane proteome of Enterococcus faecalis
Stockholm University, Faculty of Science, Department of Analytical Chemistry.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Show others and affiliations
2011 (English)In: Proteomics, ISSN 1615-9853, E-ISSN 1615-9861, Vol. 11, no Special issue, 3935-3941 p.Article in journal (Refereed) Published
Abstract [en]

Enterococcus faecalis is a gram-positive bacterium that is part of the indigenous microbiotica of humans and animals as well as an opportunistic pathogen. In this study, we have fractionated the membrane proteome of E. faecalis and identified many of its constituents by mass spectrometry. We present blue native-/SDS-PAGE reference maps that contain 102 proteins. These proteins are important for cellular homeostasis, virulence, and antibiotic intervention. Intriguingly, many proteins with no known function were also identified, indicating that there are substantial gaps in the knowledge of this organism's biology. On a more limited scale, we also provide insight into the composition of membrane protein complexes. This study is a first step toward elucidating the membrane proteome of E. faecalis, which is critical for a better understanding of how this bacterium interacts with a host and with the extracellular milieu.

Place, publisher, year, edition, pages
2011. Vol. 11, no Special issue, 3935-3941 p.
Keyword [en]
Blue native-PAGE; Enterococcus faecalis; Membrane proteome; Microbiology; Nano-LC-ESI-MS/MS; Protein complex
National Category
Analytical Chemistry
URN: urn:nbn:se:su:diva-65507DOI: 10.1002/pmic.201100103ISI: 000296142100019OAI: diva2:463976
Available from: 2011-12-12 Created: 2011-12-12 Last updated: 2012-01-31Bibliographically approved
In thesis
1. Strategies to explore the membrane proteome of a cell
Open this publication in new window or tab >>Strategies to explore the membrane proteome of a cell
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The cell envelope plays key roles in numerous processes such as maintaining cellular integrity, communication with other cells, signal transduction, maintenance of cellular homeostasis, and regulation of the traffic of molecules between the cell and the extracellular milieu. Essential membrane components in many of these processes are proteins. It is estimated that ~20-30% of the predicted open reading frames (ORFs) of all organisms encode membrane proteins. Furthermore, two thirds of drug targets are membrane proteins. However, despite their importance, membrane proteins have so far been mostly neglected in most proteomic studies, due to the inherent challenges in analyzing them.

The focus of this thesis is to devise strategies that allow investigation of membrane proteins and their associated complexes. Optimization of sample preparation in the underlying studies has allowed important goals to be reached in membrane protein analyses at various levels such as elucidation of their primary structure by collision-induced dissociation (CID) and electron-capture dissociation (ECD) mass spectrometry (MS), profiling membrane proteins and their complexes, the discovery of novel protein complexes, definition of their topology, and unambiguous identification of protein-bound ligand(s). This thesis paves the way for better characterization of membrane proteins and their assemblies hinting towards the crucial role(s) they play in maintaining normal cell physiology.

Place, publisher, year, edition, pages
Stockholm: Department of Analytical Chemistry, Stockholm University, 2011. 94 p.
Membrane proteins, Proteomics, Escherichia coli, Enterococcus faecalis, Synechocystis, Mass Spectrometry, BN/SDS-PAGE, FT-ICR-MS, Myelin P2 protein
National Category
Analytical Chemistry
Research subject
Analytical Chemistry
urn:nbn:se:su:diva-56848 (URN)978-91-7447-211-0 (ISBN)
Public defence
2011-06-08, Magnélisalen, Kemiska övningslaboratoriet, Svante Arrhenius väg 16 B, Stockholm, 10:00 (English)
At the time of the doctoral defense, the following paper was unpublished and had a status as follows: Paper 3: Manuscript. Available from: 2011-05-12 Created: 2011-04-28 Last updated: 2012-01-24Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Maddalo, GianlucaStenberg-Bruzell, FilippaIlag, LeopoldDaley, Daniel
By organisation
Department of Analytical ChemistryDepartment of Biochemistry and Biophysics
In the same journal
Analytical Chemistry

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 42 hits
ReferencesLink to record
Permanent link

Direct link