Change search
ReferencesLink to record
Permanent link

Direct link
Gene-chip studies of adipogenesis-regulated microRNAs in mouse primary adipocytes and human obesity
Stockholm University, Faculty of Science, The Wenner-Gren Institute , Physiology.
Show others and affiliations
2011 (English)In: BMC Endocrine Disorders, ISSN 1472-6823, Vol. 11, 7- p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Adipose tissue abundance relies partly on the factors that regulate adipogenesis, i.e. proliferation and differentiation of adipocytes. While components of the transcriptional program that initiates adipogenesis is well-known, the importance of microRNAs in adipogenesis is less well studied. We thus set out to investigate whether miRNAs would be actively modulated during adipogenesis and obesity.

METHODS: Several models exist to study adipogenesis in vitro, of which the cell line 3T3-L1 is the most well known, albeit not the most physiologically appropriate. Thus, as an alternative, we produced EXIQON microarray of brown and white primary murine adipocytes (prior to and following differentiation) to yield global profiles of miRNAs.

RESULTS: We found 65 miRNAs regulated during in vitro adipogenesis in primary adipocytes. We evaluated the similarity of our responses to those found in non-primary cell models, through literature data-mining. When comparing primary adipocyte profiles, with those of cell lines reported in the literature, we found a high degree of difference in 'adipogenesis' regulated miRNAs suggesting that the model systems may not be accurately representing adipogenesis. The expression of 10 adipogenesis-regulated miRNAs were studied using real-time qPCR and then we selected 5 miRNAs, that showed robust expression, were profiled in subcutaneous adipose tissue obtained from 20 humans with a range of body mass indices (BMI, range = 21-48, and all samples have U133+2 Affymetrix profiles provided). Of the miRNAs tested, mir-21 was robustly expressed in human adipose tissue and positively correlated with BMI (R2 = 0.49, p < 0.001).

CONCLUSION: In conclusion, we provide a preliminary analysis of miRNAs associated with primary cell in vitro adipogenesis and demonstrate that the inflammation-associated miRNA, mir-21 is up-regulated in subcutaneous adipose tissue in human obesity. Further, we provide a novel transcriptomics database of EXIQON and Affymetrix adipocyte profiles to facilitate data mining.

Place, publisher, year, edition, pages
2011. Vol. 11, 7- p.
Keyword [en]
primary white and brown adipocytes microRNAs; microarray; EXIQON; Affymetrix; Adipose tissue: adipocyte; transcriptome
National Category
URN: urn:nbn:se:su:diva-65634DOI: 10.1186/1472-6823-11-7ISI: 000297289500001PubMedID: 21426570OAI: diva2:464317
Available from: 2011-12-13 Created: 2011-12-13 Last updated: 2012-01-29Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Petrovic, NatasaNedergaard, JanCannon, Barbara
By organisation
In the same journal
BMC Endocrine Disorders

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 145 hits
ReferencesLink to record
Permanent link

Direct link