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Anoxia-induced elevation of cytosolic Ca2+ concentration depends on different Ca2+ sources in rice and wheat protoplasts
(Sylvia Lindberg)
St Petersburg State University. (Sylvia Lindberg)
St Petersburg State University. (TV Chirkova)
Stockholm University, Faculty of Science, Department of Botany. (Sylvia Lindberg)
2011 (English)In: Planta, Vol. 234, 271-280 p.Article in journal (Refereed) Published
Abstract [en]

The anoxia-dependent elevation of cytosolicCa2+ concentration, [Ca2+]cyt, was investigated in plants differing in tolerance to hypoxia. The [Ca2+]cyt was measuredby fluorescence microscopy in single protoplastsloaded with the calcium-fluoroprobe Fura 2-AM. Imposition of anoxia led to a fast (within 3 min) significant elevationof [Ca2+]cyt in rice leaf protoplasts. A tenfold drop in the external Ca2+ concentration (to 0.1 mM) resulted inconsiderable decrease of the [Ca2+]cyt shift. Rice root protoplasts reacted upon anoxia with higher amplitude. Addition of plasma membrane (verapamil, La3+ and EGTA) and intracellular membrane Ca2+-channel antagonists(Li+, ruthenium red and cyclosporine A) reduced the anoxic Ca2+-accumulation in rice. Wheat protoplasts responded to anoxia by smaller changes of [Ca2+]cyt. In wheat leaf protoplasts, the amplitude of the Ca2+-shift little depended on the external level of Ca2+. Wheat root protoplasts were characterized by a small shift of [Ca2+]cyt under anoxia. Plasmalemma Ca2+-channel blockers had little effect on the elevation of cytosolic Ca2+ in wheat protoplasts. Intact rice seedlings absorbed Ca2+ from the external medium under anoxic treatment. On the contrary,wheat seedlings were characterized by leakage of Ca2+. Verapamil abolished the Ca2?+influx in rice roots and Ca2+ efflux from wheat roots. Anoxia-induced [Ca2+]cyt elevation was high particularly in rice, a hypoxia-tolerant species. In conclusion, both external and internal Ca2+ stores are important for anoxic [Ca2+]cyt elevation in rice, whereas the hypoxia-intolerant wheat does not requireexternal sources for [Ca2+]cyt rise. Leaf and root protoplasts similarly responded to anoxia, independent of theirorgan origin.

Place, publisher, year, edition, pages
Springer, 2011. Vol. 234, 271-280 p.
Keyword [en]
Anoxic signalling, cytosolic calcium, Fluorescence microscopy, Oryza, Triticum
National Category
Natural Sciences Botany
Research subject
Plant Physiology
URN: urn:nbn:se:su:diva-66075DOI: 10.1007/s00425-011-1396-xISI: 000293188600005OAI: diva2:466844
Calcium signalling under anoxia
authorCount :4Available from: 2011-12-16 Created: 2011-12-16 Last updated: 2012-01-12Bibliographically approved

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