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Metabolite profiling of nitrogen-fixing actinorhizal plants, Casuarina glauca and Datisca glomerata: Master’s Degree Thesis project ‐ 45 hp
Stockholm University, Faculty of Science, Department of Botany. (Katharina Pawlowski/Thomas Fester)
2011 (English)Independent thesis Advanced level (degree of Master (Two Years)), 30 credits / 45 HE creditsStudent thesis
Abstract [en]

Casuarina glauca and Datisca glomerata are two actinorhizal plants, able to enter a mutualisticroot‐symbiosis with the nitrogen‐fixing actinobacteria of the genus Frankia. Under soilconditions with low ammonium content, actinorhizal plants produce novel organs called rootnodules,which provide a niche to the bacteria. Formation of root‐nodules is a highly‐regulatedprocess involving the exchange of specific signals and changes in plant morphology andmetabolism. In this project we studied metabolic characteristics of nodules from C. glauca and D. glomerata combined with metabolic changes induced by salt‐stress. We were interested inparticular in possible reciprocal effects of the salt‐stress and the symbiotic interaction in a saltsensitive(D. glomerata) and a salt‐tolerant (C. glauca) plant. Metabolite profiling was pursuedusing a gas‐chromatography‐mass‐spectrometry (GC‐MS) approach. Metabolite profilesobtained from the analysis of different plant organs ‐ leaves, nodules and roots – were analyzedfor differences induced by salt treatments. In addition metabolite profiles of roots and nodules(under salt‐ and non‐salt conditions) were compared with each other. While the analysis ofbelowground tissues was focused on metabolic changes induced by the treatments on a locallevel, systemic effects were referred to by respective analyses of leaves. Results show thedistinct metabolisms between roots and nodules organs and the increase in their differencesunder salt‐stress conditions. Nodulated plants also appeared more influenced by salt‐stressthan non‐nodulated ones. Moreover, belowground organs interactions with Frankia bacteriaand/or salt‐stress produced metabolic changes which were detected also in leaves, as result of asystemic effect.

Place, publisher, year, edition, pages
2011. , 54 p.
National Category
Natural Sciences
URN: urn:nbn:se:su:diva-66081OAI: diva2:466898
External cooperation
Thomas Fester, UFZ Leipzig, Germany
2011-11-11, Botan 540, Lilla Frescativägen 5, Stockholm, 11:11 (English)
Life Earth Science
Available from: 2012-01-10 Created: 2011-12-17 Last updated: 2012-01-10Bibliographically approved

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