beta-Adrenergic Inhibition of Contractility in L6 Skeletal Muscle Cells
2011 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 6, no 7, e22304- p.Article in journal (Refereed) Published
The beta-adrenoceptors (beta-ARs) control many cellular processes. Here, we show that beta-ARs inhibit calcium depletion-induced cell contractility and subsequent cell detachment of L6 skeletal muscle cells. The mechanism underlying the cell detachment inhibition was studied by using a quantitative cell detachment assay. We demonstrate that cell detachment induced by depletion of extracellular calcium is due to myosin-and ROCK-dependent contractility. The beta-AR inhibition of L6 skeletal muscle cell detachment was shown to be mediated by the beta(2)-AR and increased cAMP but was surprisingly not dependent on the classical downstream effectors PKA or Epac, nor was it dependent on PKG, PI3K or PKC. However, inhibition of potassium channels blocks the beta(2)-AR mediated effects. Furthermore, activation of potassium channels fully mimicked the results of beta(2)-AR activation. In conclusion, we present a novel finding that beta(2)-AR signaling inhibits contractility and thus cell detachment in L6 skeletal muscle cells by a cAMP and potassium channel dependent mechanism.
Place, publisher, year, edition, pages
2011. Vol. 6, no 7, e22304- p.
IdentifiersURN: urn:nbn:se:su:diva-66565DOI: 10.1371/journal.pone.0022304ISI: 000293284600009OAI: oai:DiVA.org:su-66565DiVA: diva2:468923
authorCount :32011-12-222011-12-202013-01-30Bibliographically approved