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Shift in Ribonucleotide Reductase Gene Expression in Pseudomonas aeruginosa during Infection
Stockholm University, Faculty of Science, Department of Molecular Biology and Functional Genomics.
Stockholm University, Faculty of Science, Department of Molecular Biology and Functional Genomics.
2011 (English)In: Infection and Immunity, ISSN 0019-9567, E-ISSN 1098-5522, Vol. 79, no 7, 2663-2669 p.Article in journal (Refereed) Published
Abstract [en]

The roles of different ribonucleotide reductases (RNRs) in bacterial pathogenesis have not been studied systematically. In this work we analyzed the importance of the different Pseudomonas aeruginosa RNRs in pathogenesis using the Drosophila melanogaster host-pathogen interaction model. P. aeruginosa codes for three different RNRs with different environmental requirements. Class II and III RNR chromosomal mutants exhibited reduced virulence in this model. Translational reporter fusions of RNR gene nrdA, nrdJ, or nrdD to the green fluorescent protein were constructed to measure the expression of each class during the infection process. Analysis of the P. aeruginosa infection by flow cytometry revealed increased expression of nrdJ and nrdD and decreased nrdA expression during the infection process. Expression of each RNR class fits with the pathogenicities of the chromosomal deletion mutants. An extended understanding of the pathogenicity and physiology of P. aeruginosa will be important for the development of novel drugs against infections in cystic fibrosis patients.

Place, publisher, year, edition, pages
2011. Vol. 79, no 7, 2663-2669 p.
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:su:diva-66975DOI: 10.1128/IAI.01212-10ISI: 000291788700017OAI: oai:DiVA.org:su-66975DiVA: diva2:470184
Note
authorCount :2Available from: 2011-12-28 Created: 2011-12-22 Last updated: 2017-12-08Bibliographically approved

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