Change search
ReferencesLink to record
Permanent link

Direct link
Cohesin phosphorylation and mobility of SMC1 at ionizing radiation-induced DNA double-strand breaks in human cells
Show others and affiliations
2011 (English)In: Experimental Cell Research, ISSN 0014-4827, E-ISSN 1090-2422, Vol. 317, no 3, 330-337 p.Article in journal (Refereed) Published
Abstract [en]

Cohesin, a hetero-tetrameric complex of SMC1, SMC3, Rad21 and Scc3, associates with chromatin after mitosis and holds sister chromatids together following DNA replication. Following DNA damage, cohesin accumulates at and promotes the repair of DNA double-strand breaks. In addition, phosphorylation of the SMC1/3 subunits contributes to DNA damage-induced cell cycle checkpoint regulation. The aim of this study was to determine the regulation and consequences of SMC1/3 phosphorylation as part of the cohesin complex. We show here that the ATM-dependent phosphorylation of SMC1 and SMC3 is mediated by H2AX, 53BP1 and MDC1. Depletion of RAD21 abolishes these phosphorylations, indicating that only the fully assembled complex is phosphorylated. Comparison of wild type SMC1 and SMC1S966A in fluorescence recovery after photo-bleaching experiments shows that phosphorylation of SMC1 is required for an increased mobility after DNA damage in G2-phase cells, suggesting that ATM-dependent phosphorylation facilitates mobilization of the cohesin complex after DNA damage.

Place, publisher, year, edition, pages
2011. Vol. 317, no 3, 330-337 p.
Keyword [en]
ATM, SMC1, SMC3, Cohesin, Ionizing radiation, DNA repair
National Category
Cancer and Oncology
URN: urn:nbn:se:su:diva-67353DOI: 10.1016/j.yexcr.2010.10.021ISI: 000286367700007OAI: diva2:470187

authorCount :6

Available from: 2011-12-28 Created: 2011-12-28 Last updated: 2012-09-19Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Helleday, Thomas
By organisation
Department of Genetics, Microbiology and Toxicology
In the same journal
Experimental Cell Research
Cancer and Oncology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 161 hits
ReferencesLink to record
Permanent link

Direct link