Identification of Chromatophore Membrane Protein Complexes Formed under Different Nitrogen Availability Conditions in Rhodospirillum rubrum
2011 (English)In: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 10, no 6, 2703-2714 p.Article in journal (Refereed) Published
The chromatophore membrane of the photosynthetic diazotroph Rhodospirillum rubrum is of vital importance for a number of central processes, including nitrogen fixation. Using a novel amphiphile, we have identified protein complexes present under different nitrogen availability conditions by the use of two-dimensional Blue Native/SDS-PAGE and NSI-LC-LTQ-Orbitrap mass spectrometry. We have identified several membrane protein complexes, including components of the ATP synthase, reaction center, light harvesting, and NADH dehydrogenase complexes. Additionally, we have identified differentially expressed proteins, such as subunits of the succinate dehydrogenase complex and other TCA cycle enzymes that are usually found in the cytosol, thus hinting at a possible association to the membrane in response to nitrogen deficiency. We propose a redox sensing mechanism that can influence the membrane subproteome in response to nitrogen availability.
Place, publisher, year, edition, pages
2011. Vol. 10, no 6, 2703-2714 p.
Rhodospirillum rubrum, nitrogen metabolism, Blue Native, chromatophore subproteome, amphiphile, Orbitrap
IdentifiersURN: urn:nbn:se:su:diva-67875DOI: 10.1021/pr100838xISI: 000291186100002OAI: oai:DiVA.org:su-67875DiVA: diva2:471377
authorCount :82012-01-022012-01-022012-10-03Bibliographically approved