Change search
ReferencesLink to record
Permanent link

Direct link
Purification, Modeling, and Analysis of Botulinum Neurotoxin Subtype A5 (BoNT/A5) from Clostridium botulinum Strain A661222
Show others and affiliations
2011 (English)In: Applied and Environmental Microbiology, ISSN 0099-2240, E-ISSN 1098-5336, Vol. 77, no 12, 4217-4222 p.Article in journal (Refereed) Published
Abstract [en]

A Clostridium botulinum type A strain (A661222) in our culture collection was found to produce the botulinum neurotoxin subtype A5 (BoNT/A5). Its neurotoxin gene was sequenced to determine its degree of similarity to available sequences of BoNT/A5 and the well-studied BoNT/A1. Thirty-six amino acid differences were observed between BoNT/A5 and BoNT/A1, with the predominant number being located in the heavy chain. The amino acid chain of the BoNT/A from the A661222 strain was superimposed over the crystal structure of the known structure of BoNT/A1 to assess the potential significance of these differences-specifically how they would affect antibody neutralization. The BoNT/A5 neurotoxin was purified to homogeneity and evaluated for certain properties, including specific toxicity and antibody neutralization. This study reports the first purification of BoNTA5 and describes distinct differences in properties between BoNT/A5 and BoNT/A1.

Place, publisher, year, edition, pages
2011. Vol. 77, no 12, 4217-4222 p.
National Category
Biological Sciences Structural Biology Biochemistry and Molecular Biology
Research subject
Biochemistry; Structural Biology
URN: urn:nbn:se:su:diva-67871DOI: 10.1128/AEM.00201-11ISI: 000291341800036OAI: diva2:471436
Swedish Research Council, 2010–5200The Wenner-Gren FoundationSwedish Foundation for Strategic Research , ICA08-0001

authorCount :7

Available from: 2012-01-02 Created: 2012-01-02 Last updated: 2013-09-24Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Stenmark, Pål
By organisation
Department of Biochemistry and Biophysics
In the same journal
Applied and Environmental Microbiology
Biological SciencesStructural BiologyBiochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 41 hits
ReferencesLink to record
Permanent link

Direct link