Monosaccharide compositional analysis of marine polysaccharides by hydrophilic interaction liquid chromatography-tandem mass spectrometry
2011 (English)In: Analytical and Bioanalytical Chemistry, ISSN 1618-2642, E-ISSN 1618-2650, Vol. 399, no 7, 2517-2529 p.Article in journal (Refereed) Published
A simple and sensitive method was developed using hydrophilic interaction liquid chromatography coupled to tandem mass spectrometry for determination of monosaccharides liberated from marine polysaccharides by acidic hydrolysis. Optimal separation of diastereomeric monosaccharides including hexoses, pentoses, and deoxyhexoses was achieved using an aminopropyl bonded column with mobile phase containing ternary solvents (acetonitrile/methanol/water) in conjunction with MS/MS in SRM mode. Mechanisms for fragmentation of deprotonated monosaccharides with regard to cross-ring cleavage were proposed. Matrix effects from coeluting interferences were observed and isotopic-labeled internal standard was used to compensate for the signal suppression. The method demonstrated excellent instrumental limits of detection (LOD), ranging from 0.7 to 4.2 pg. Method LODs range from 0.9 to 5.1 nM. The proposed method was applied to the analysis of polysaccharides in seawater collected from the open leads of the central Arctic Ocean in the summer of 2008.
Place, publisher, year, edition, pages
2011. Vol. 399, no 7, 2517-2529 p.
Hydrophilic interaction liquid chromatography, Tandem mass spectrometry, Monosaccharides, Polysaccharides, Marine microcolloids, Marine gels
Analytical Chemistry Biochemistry and Molecular Biology
Research subject Analytical Chemistry; Biophysics
IdentifiersURN: urn:nbn:se:su:diva-67919DOI: 10.1007/s00216-010-4638-zISI: 000287141700024OAI: oai:DiVA.org:su-67919DiVA: diva2:471875
authorCount :42012-01-032012-01-022012-02-15Bibliographically approved