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Toxicity and SOS-response to ELF magnetic fields and nalidixic acid in E. coli cells
Stockholm University, Faculty of Science, Department of Genetics, Microbiology and Toxicology.
2011 (English)In: Mutation research. Genetic toxicology and environmental mutagenesis, ISSN 1383-5718, Vol. 722, no 1, 56-61 p.Article in journal (Refereed) Published
Abstract [en]

Extremely low-frequency magnetic fields (ELF-MF) have previously been shown to affect conformation of chromatin and cell proliferation. Possible genotoxic and carcinogenic effects of ELF-MF have also been discussed and tested. In this study, we analysed the effect of ELF-MF on chromatin conformation in E. call GE499 cells by the anomalous viscosity time-dependence (AVTD) technique. Possible genotoxic effects of the specific combination of static and ELF-MF, which has been proven to affect chromatin conformation, were investigated by a clonogenic assay, by assessing cell-growth kinetics, and by analysis of the SOS-response by means of inducible recA-lacZ fusion-gene products and the beta-galactosidase assay. The genotoxic agent nalidixic acid (NAL) was used as a positive control and in combination with ELF-MF. Nalidixic acid at 3-30 mu g/ml decreased the AVTD peaks and induced a cytotoxic effect. In contrast to NAL, ELF-MF fields increased AVTD, stimulated cell growth, and increased cloning efficiency. These effects depended on the frequency within the range of 7-11 Hz. While NAL induced an SOS-response, exposure to ELF-MF did not induce the recA-lacZ fusion-gene product. Exposure to ELF-MF did not modify the genotoxic effects of NAL either. All together, the data show that ELF-MF, under specific conditions of exposure, acted as a non-toxic but cell-growth stimulating agent.

Place, publisher, year, edition, pages
2011. Vol. 722, no 1, 56-61 p.
Keyword [en]
ELF magnetic field, Genotoxicity, recA-lacZ fusion, SOS-response
National Category
Biological Sciences
URN: urn:nbn:se:su:diva-68094DOI: 10.1016/j.mrgentox.2011.03.012ISI: 000291913600008OAI: diva2:472662
authorCount :1Available from: 2012-01-04 Created: 2012-01-03 Last updated: 2012-01-04Bibliographically approved

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