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Novel Affinity Ligands for Chromatography Using Combinatorial Chemistry
Stockholm University, Faculty of Science, Department of Organic Chemistry.
Stockholm University, Faculty of Science, Department of Organic Chemistry.
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2011 (English)In: Combinatorial chemistry & high throughput screening, ISSN 1386-2073, E-ISSN 1875-5402, Vol. 14, no 4, 267-278 p.Article in journal (Refereed) Published
Abstract [en]

Spatially addressable combinatorial libraries were synthesized by solution phase chemistry and screened for binding to human serum albumin. Members of arylidene diamide libraries were among the best hits found, having submicromolar binding affinities. The results were analyzed by the frequency with which particular substituents appeared among the most potent compounds. After immobilization of the ligands either through the oxazolone or the amine substituent, characterization by surface plasmon resonance showed that ibuprofen affected the binding kinetics, but phenylbutazone did not. It is therefore likely that these compounds bind to Site 2 in sub domain IIIA of human serum albumin (HSA).

Place, publisher, year, edition, pages
2011. Vol. 14, no 4, 267-278 p.
Keyword [en]
Affinity chromatography, combinatorial chemistry, high-throughput screening, arylidene diamide library
National Category
Organic Chemistry
URN: urn:nbn:se:su:diva-68543DOI: 10.2174/138620711795222482ISI: 000290611700004OAI: diva2:472724

authorCount :9

Available from: 2012-01-04 Created: 2012-01-04 Last updated: 2015-10-16Bibliographically approved

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Pilotti, Åke
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Department of Organic Chemistry
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