Change search
ReferencesLink to record
Permanent link

Direct link
A cis-regulatory map of the Drosophila genome
Show others and affiliations
2011 (English)In: Nature, ISSN 0028-0836, Vol. 471, no 7339, 527-531 p.Article in journal (Refereed) Published
Abstract [en]

Systematic annotation of gene regulatory elements is a major challenge in genome science. Direct mapping of chromatin modification marks and transcriptional factor binding sites genome-wide(1,2) has successfully identified specific subtypes of regulatory elements(3). In Drosophila several pioneering studies have provided genome-wide identification of Polycomb response elements(4), chromatin states(5), transcription factor binding sites(6-9), RNA polymerase II regulation(8) and insulator elements(10); however, comprehensive annotation of the regulatory genome remains a significant challenge. Here we describe results from the modENCODE cis-regulatory annotation project. We produced a map of the Drosophila melanogaster regulatory genome on the basis of more than 300 chromatin immunoprecipitation data sets for eight chromatin features, five histone deacetylases and thirty-eight site-specific transcription factors at different stages of development. Using these data we inferred more than 20,000 candidate regulatory elements and validated a subset of predictions for promoters, enhancers and insulators in vivo. We identified also nearly 2,000 genomic regions of dense transcription factor binding associated with chromatin activity and accessibility. We discovered hundreds of new transcription factor co-binding relationships and defined a transcription factor network with over 800 potential regulatory relationships.

Place, publisher, year, edition, pages
2011. Vol. 471, no 7339, 527-531 p.
National Category
Biological Sciences
URN: urn:nbn:se:su:diva-69110DOI: 10.1038/nature09990ISI: 000288702200065OAI: diva2:474896
authorCount :40Available from: 2012-01-10 Created: 2012-01-10 Last updated: 2012-01-10Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Mannervik, Mattias
By organisation
Developmental Biology
In the same journal
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 209 hits
ReferencesLink to record
Permanent link

Direct link