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Characterization of nifH gene expression, modification and rearrangement in Nodularia spumigena strain AV1
Stockholm University, Faculty of Science, Department of Botany.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Botany.
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2011 (English)In: FEMS Microbiology Ecology, ISSN 0168-6496, E-ISSN 1574-6941, Vol. 77, no 2, 449-459 p.Article in journal (Refereed) Published
Abstract [en]

The annually reoccurring blooms that characterize the surface waters of the Baltic Sea are dominated by filamentous, heterocystous cyanobacteria such as Nodularia spumigena. In a previous study, we have demonstrated that N. spumigena strain AV1 differentiates heterocysts in the absence of detectable nitrogen fixation activity, an unusual physiological trait that is clearly distinct from other well-studied cyanobacteria. To further analyze the uncoupling between these two processes, we analyzed the gene expression and modification of the nitrogenase enzyme (the enzyme responsible for nitrogen fixation) in N. spumigena AV1, as well as in several other N. spumigena strains. Here, we demonstrate the occurrence of two nifH gene copies in N. spumigena strain AV1, only one of which is located in a complete nifHDK cluster and several NifH protein forms. Furthermore, we demonstrate the occurrence of a DNA rearrangement mechanism acting within the nifH gene copy located in the nifHDK cluster and present only in the strains exhibiting the previously reported uncoupling between heterocyst differentiation and nitrogen fixation processes. These data stress the existence of a distinct and complex regulatory circuit related to nitrogen fixation in this ecologically significant bloom-forming cyanobacterium.

Place, publisher, year, edition, pages
2011. Vol. 77, no 2, 449-459 p.
Keyword [en]
Nodularia spumigena, nitrogenase, nitrogen fixation, gene rearrangement, post-translational modification, cyanobacteria
National Category
Microbiology Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:su:diva-69261DOI: 10.1111/j.1574-6941.2011.01127.xISI: 000292608000020OAI: oai:DiVA.org:su-69261DiVA: diva2:476442
Note
authorCount :5Available from: 2012-01-12 Created: 2012-01-11 Last updated: 2017-12-08Bibliographically approved

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