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Uptake kinetics of cell-penetrating peptides
Stockholm University, Faculty of Science, Department of Neurochemistry. Tartu University, Estonia.ORCID iD: 0000-0001-6107-0844
2011 (English)In: Cell-penetrating peptides: Methods and Protocols, Humana Press, 2011, p. 117-128Chapter in book (Refereed)
Abstract [en]

As our knowledge increases about the diversity in uptake mechanisms displayed by cell-penetrating peptides (CPP), the concept of CPP uptake kinetics becomes increasingly complex. Here, we present three different assays that can be used for studying different kinetic aspects of CPP-mediated delivery: intracellular accumulation and membranolytical effects, intracellular CPP-cargo detachment, and finally a functional readout of a biological action from the delivered cargo. Unlike the traditional end-point measurements that give a static postincubation readout, these assays are all dynamic, real-time, in situ measurements obtained during incubation. A combination of some (or all) of these different assays gives us not only interesting kinetic information about the uptake routes but also provides a simple and valuable methodology for the evaluation of potential drug candidates based on the chemical modification of CPPs by cargo attachment.

Place, publisher, year, edition, pages
Humana Press, 2011. p. 117-128
Series
Methods in Molecular Biology, ISSN 1064-3745 ; 683
Keyword [en]
cpp, cell-penetrating peptides, transfection
National Category
Neurosciences Chemical Sciences Other Chemistry Topics
Research subject
Neurochemistry with Molecular Neurobiology
Identifiers
URN: urn:nbn:se:su:diva-70607DOI: 10.1007/978-1-60761-919-2ISBN: 978-1-60761-918-5 (print)ISBN: 978-1-60761-919-2 (print)OAI: oai:DiVA.org:su-70607DiVA, id: diva2:482109
Available from: 2012-01-23 Created: 2012-01-23 Last updated: 2018-01-12Bibliographically approved

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