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Uptake kinetics of cell-penetrating peptides
Stockholm University, Faculty of Science, Department of Neurochemistry. Tartu University, Estonia.ORCID iD: 0000-0001-6107-0844
2011 (English)In: Cell-penetrating peptides: Methods and Protocols, Humana Press, 2011, 117-128 p.Chapter in book (Refereed)
Abstract [en]

As our knowledge increases about the diversity in uptake mechanisms displayed by cell-penetrating peptides (CPP), the concept of CPP uptake kinetics becomes increasingly complex. Here, we present three different assays that can be used for studying different kinetic aspects of CPP-mediated delivery: intracellular accumulation and membranolytical effects, intracellular CPP-cargo detachment, and finally a functional readout of a biological action from the delivered cargo. Unlike the traditional end-point measurements that give a static postincubation readout, these assays are all dynamic, real-time, in situ measurements obtained during incubation. A combination of some (or all) of these different assays gives us not only interesting kinetic information about the uptake routes but also provides a simple and valuable methodology for the evaluation of potential drug candidates based on the chemical modification of CPPs by cargo attachment.

Place, publisher, year, edition, pages
Humana Press, 2011. 117-128 p.
Series
Methods in Molecular Biology, ISSN 1064-3745 ; 683
Keyword [en]
cpp, cell-penetrating peptides, transfection
National Category
Neurosciences Chemical Sciences Other Chemistry Topics
Research subject
Neurochemistry with Molecular Neurobiology
Identifiers
URN: urn:nbn:se:su:diva-70607DOI: 10.1007/978-1-60761-919-2ISBN: 978-1-60761-918-5 (print)ISBN: 978-1-60761-919-2 (print)OAI: oai:DiVA.org:su-70607DiVA: diva2:482109
Available from: 2012-01-23 Created: 2012-01-23 Last updated: 2015-04-21Bibliographically approved

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