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Characterization of cellular internalization pathways for CPP-mediated oligonucleotide delivery
Stockholm University, Faculty of Science, Department of Neurochemistry.
Stockholm University, Faculty of Science, Department of Neurochemistry.
Stockholm University, Faculty of Science, Department of Neurochemistry.ORCID iD: 0000-0001-6107-0844
2011 (English)In: Cell-penetrating peptides: Methods and Protocols / [ed] Ülo Langel, New York: Humana Press, 2011, 219-230 p.Chapter in book (Other academic)
Abstract [en]

The methods for evaluating internalization pathways of cellular CPP-mediated ON delivery utilizing a pre-mRNA splice correction assay and fluorescence-based quantification are described. Examples for characterization of CPP uptake routes, employing various endocytosis inhibitors, and special treatment conditions are demonstrated. The methods are developed to characterize cellular delivery of pre-mRNA splice switching peptide nucleic acids conjugated to CPPs by disulfide bond.

Place, publisher, year, edition, pages
New York: Humana Press, 2011. 219-230 p.
Series
Methods in Molecular Biology, ISSN 1064-3745 ; 683
Keyword [en]
cpp, cell-penetrating peptides, transfection, Pre-mRNA splicing, Oligonucleotide delivery, Internalization pathways, Endocytosis inhibitors
National Category
Neurosciences Cell and Molecular Biology Other Chemistry Topics Biochemistry and Molecular Biology
Research subject
Neurochemistry with Molecular Neurobiology
Identifiers
URN: urn:nbn:se:su:diva-70614DOI: 10.1007/978-1-60761-919-2ISBN: 978-1-60761-918-5 (print)OAI: oai:DiVA.org:su-70614DiVA: diva2:482131
Available from: 2012-01-23 Created: 2012-01-23 Last updated: 2015-04-21Bibliographically approved

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