Change search
ReferencesLink to record
Permanent link

Direct link
Cell-penetrating peptides-based strategies for the delivery of splice redirecting antisense oligonucleotides
Stockholm University, Faculty of Science, Department of Neurochemistry.
Université Montpellier, Motpellier, France.
Université Montpellier, Motpellier, France.
University of Tartu, Institute of Technology, Tartu, Estonia.
Show others and affiliations
2011 (English)In: Therapeutic Oligonucleotides: Methods and Protocols / [ed] John Goodchild, New York: Humana Press, 2011, 75-89 p.Chapter in book (Other academic)
Abstract [en]

Progress in our understanding of the molecular pathogenesis of human malignancies has provided therapeutic targets amenable to oligonucleotide (ON)-based strategies. Antisense ON-mediated splicing regulation in particular offers promising prospects since the majority of human genes undergo alternative splicing and since splicing defects have been found in many diseases. However, their implementation has been hampered so far by the poor bioavailability of nucleic acids-based drugs. Cell-penetrating peptides (CPPs) now appear as promising non-viral delivery vector for non-permeant biomolecules. We describe here new CPPs allowing the delivery of splice redirecting steric-block ON using either chemical conjugation or non-covalent complexation. We also describe a convenient and robust splice redirecting assay which allows the quantitative assessment of ON nuclear delivery.

Place, publisher, year, edition, pages
New York: Humana Press, 2011. 75-89 p.
, Methods in Molecular Biology, ISSN 1064-3745 ; 764
Keyword [en]
cpp, cell-penetrating peptides, transfection
National Category
Cell and Molecular Biology Chemical Sciences Other Chemistry Topics Biochemistry and Molecular Biology
Research subject
Neurochemistry with Molecular Neurobiology
URN: urn:nbn:se:su:diva-70620DOI: 10.1007/978-1-61779-188-8_5OAI: diva2:482181
Available from: 2012-01-23 Created: 2012-01-23 Last updated: 2015-04-21Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Langel, Ülo
By organisation
Department of Neurochemistry
Cell and Molecular BiologyChemical SciencesOther Chemistry TopicsBiochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 29 hits
ReferencesLink to record
Permanent link

Direct link