Antimicrobial activity of enantiomeric pairs of cationic cyclic lipopeptides against Staphylococcus aureus
(English)Manuscript (preprint) (Other academic)
We have previously shown that the peptides cyclo[D-Tle-D-Lys-D-Tle-L-Ala-D-Tle-L-Ala-D-Tle-L-Ala] and cyclo[L-Tle-L-Lys-L-Tle-D-Ala-L-Tle-D-Ala-L-Tle-D-Ala] have a potent antimicrobial activity against Staphylococcus aureus when combined but almost no effect as a single peptide. In this study the antimicrobial activity of the peptides cyclo L-Lys(R)-L-Tle-D-Ala-L-Tle-D-Ala-L-Tle-D-Ala-L-Tle and cyclo D-Lys(R)-D-Tle-L-Ala-D-Tle-L-Ala-D-Tle-L-Ala-D-Tle where R is –L-Arg-L-Arg-L-Arg-CO-(CH2)6-CH3 was investigated against Staphylococcus aureus in a broth microdilution assay. At a concentration of 10 mM neither peptide showed any antimicrobial activity. However, when both peptides were present in the experiment a potent inhibition with a MIC value of 8.9 mM was observed. These results show that this class of peptides can be modified by branching of the side-chain of an amino acid residue in the cyclic peptide while retaining or possibly enhancing the antimicrobial activity. This observation will facilitate the optimization of antimicrobial activity and specificity of peptides with similar design.
Research subject Neurochemistry with Molecular Neurobiology
IdentifiersURN: urn:nbn:se:su:diva-72967OAI: oai:DiVA.org:su-72967DiVA: diva2:504650