Elizabethkingia anophelis sp nov., isolated from the midgut of the mosquito Anopheles gambiae
2011 (English)In: International Journal of Systematic and Evolutionary Microbiology, ISSN 1466-5026, E-ISSN 1466-5034, Vol. 61, 2670-2675 p.Article in journal (Refereed) Published
The taxonomic position, growth characteristics and antibiotic resistance properties of a slightly yellow-pigmented bacterial strain, designated R26(T), isolated from the midgut of the mosquito Anopheles gambiae, were studied. The isolate produced rod-shaped cells, which stained Gram-negative. The bacterium had two growth optima at 30-31 degrees C and 37 degrees C. Strain R26(T) demonstrated natural antibiotic resistance to ampicillin, chloramphenicol, kanamycin, streptomycin and tetracycline. 16S rRNA gene sequence analysis revealed that the isolate showed 98.6% sequence similarity to that of Elizabethkingia meningoseptica ATCC 13253(T) and 98.2 % similarity to that of Elizabethkingia miricola GTC 862(T). The major fatty acids of strain R26(T) were iso-C(15:0), iso-C(17:0) 3-OH and summed feature 4 (iso-C(15:0) 2-OH and/or C(16:1)omega 7c/t. Strain R26(T) contained only menaquinone MK-6 and showed a complex polar lipid profile consisting of diphosphatidylglycerol, phosphatidylinositol, an unknown phospholipid and unknown polar lipids and glycolipids. DNA-DNA hybridization experiments with E. meningoseptica CCUG 214(T) (=ATCC 13253(T)) and E. miricola KCTC 12492(T) (=GTC 862(T)) gave relatedness values of 34.5 % (reciprocal 41.5 %) and 35.0 % (reciprocal 25.7 %), respectively. DNA-DNA hybridization results and some differentiating biochemical properties indicate that strain R26T represents a novel species, for which the name Elizabethkingia anophelis sp. nov. is proposed. The type strain is R26(T) (=CCUG 60038(T) =CCM 7804(T)).
Place, publisher, year, edition, pages
2011. Vol. 61, 2670-2675 p.
IdentifiersURN: urn:nbn:se:su:diva-74057DOI: 10.1099/ijs.0.026393-0ISI: 000297453500019OAI: oai:DiVA.org:su-74057DiVA: diva2:506421
authorCount :62012-02-282012-02-282012-02-28Bibliographically approved