Change search
ReferencesLink to record
Permanent link

Direct link
Tape-stripping as a method for measuring dermal exposure to resin acids during wood pellet production
Department of Occupational and Environmental Medicine, University Hospital of Northern Sweden, Umeå, Sweden.
Department of Environmental Science, Örebro University, Örebro, .
Örebro Univ Hosp, Dept Occupat & Environm Med.
Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA .
2008 (English)In: Journal of Environmental Monitoring, ISSN 1464-0325, E-ISSN 1464-0333, Vol. 10, 345-352 p.Article in journal (Refereed) Published
Abstract [en]

The purpose of this study was to develop a sensitive and specific method for quantifying dermal exposure to the resin acids 7-oxodehydroabietic acid (7-OXO), dehydroabietic acid (DHAA), abietic acid (AA), and pimaric acid (PA). In addition the method was evaluated in occupational settings during production of wood pellets. Tape-strips were spiked with the substances to evaluate the recovery of the acids from the tape. The removal efficiency of the tape was assessed by tape-stripping a specified area on a glass plate spiked with resin acids. The recovery of the acids from human skin in vivo was evaluated by applying acids in methanol onto the skin of volunteers. Occupational dermal exposure to the resin acids was assessed by tape-stripping the skin of workers involved in the production of wood pellets. The resin acids were analyzed by liquid chromatography mass spectrometry (LC-MS). The limit of detection was 15 pg (7-OXO), 150 pg (DHAA), 285 pg (AA) and 471 pg (PA) per injection. The recovery from spiked tapes was in general 100%. The removal efficiency of the tape was 48–101%. Recovery tests from human skin in vivo showed a mean recovery of 27%. Quantifiable amounts of resin acids were observed on four different skin areas with an increase in exposure during a work shift. This study shows that occupational dermal exposure to resin acids can be assessed by tape-stripping and quantified by LC-MS.

Place, publisher, year, edition, pages
2008. Vol. 10, 345-352 p.
National Category
Analytical Chemistry Other Medical Sciences not elsewhere specified
URN: urn:nbn:se:su:diva-74450DOI: 10.1039/b719152aISI: 000253908800009OAI: diva2:509356
Available from: 2012-03-12 Created: 2012-03-12 Last updated: 2012-07-24Bibliographically approved
In thesis
1. Resin acids in commercial products and the work environment of Swedish wood pellets production: Analytical methodology, occurrence and exposure
Open this publication in new window or tab >>Resin acids in commercial products and the work environment of Swedish wood pellets production: Analytical methodology, occurrence and exposure
2012 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The aims of the work this thesis is based upon were to develop convenient analytical procedures for determining resin acids in biological and environmental matrices, and apply them to enhance understanding of the occurrence, exposure to and uptake by exposed individuals of resin acids. Particular focus has been on the workplace environment of the Swedish wood pellets industry. Sample extraction procedures and high-performance liquid chromatography/electrospray ionisation-mass spectrometry (HPLC/ESI-MS) methodologies were developed for measuring resin acids in dust, skin and urine samples. Chromatographic separation of abietic (AA) and pimaric acid was achieved by using a polar-embedded C12 stationary phase. The HPLC/ESI-MS method avoids undesirable oxidation of AA, which was found to occur during the derivatisation step in the standard MDHS 83/2 gas chromatography/flame ionisation detection (GC/FID) methodology, leading to false observations of both AA and the oxidation product 7-oxodehydroabietic acid (7-OXO). Personal exposures to resin acids in the Swedish wood pellet production industry were found to be lower, on average, than the British Occupational Exposure Limit for rosin (50 µg/m3). The oxidised resin acid 7-OXO, was detected in both dust and skin samples indicating the presence of allergenic resin acids. A correlation between air and post-shift urinary concentrations of dehydroabietic acid (DHAA), and a trend towards an increase in urinary 7-OXO during work shifts, were also observed. Whether the increase in 7-OXO was due to direct uptake or metabolism of other resin acids cannot be concluded from the results. An efficient HPLC/UV methodology with diode-array detection was developed for screening commercial products for rosin that could be used in laboratories lacking mass spectrometers. Very high concentrations of free resin acids were detected in depilatory wax strips using the method.

Place, publisher, year, edition, pages
Stockholm: Department of Analytical Chemistry, Stockholm University, 2012. 60 p.
Rosin, resin acids, HPLC/ESI-MS, air sampling, urine samples, wood pellets, wood dust
National Category
Analytical Chemistry
Research subject
Analytical Chemistry
urn:nbn:se:su:diva-74448 (URN)978-91-7447-449-7 (ISBN)
Public defence
2012-04-27, Magnélisalen, Kemiska övningslaboratoriet, Svante Arrhenius väg 16 B, Stockholm, 13:00 (English)
At the time of doctoral defense, the following paper was unpublished and had a status as follows: Paper 4: Submitted.Available from: 2012-04-03 Created: 2012-03-12 Last updated: 2012-03-28Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text
In the same journal
Journal of Environmental Monitoring
Analytical ChemistryOther Medical Sciences not elsewhere specified

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 81 hits
ReferencesLink to record
Permanent link

Direct link