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HPLC/neg-ESI-MS determination of resin acids in urine from Swedish wood pellets production plants workers and correlation with air concentrations
Stockholm University, Faculty of Science, Department of Analytical Chemistry.
Department of Occupational and Environmental Medicine, University Hospital of Northern Sweden, Umeå, Sweden.
Department of Environmental Science, Örebro University, Örebro.
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2012 (English)In: Analytical and Bioanalytical Chemistry, ISSN 1618-2642, E-ISSN 1618-2650Article in journal (Refereed) Submitted
Place, publisher, year, edition, pages
National Category
Analytical Chemistry Other Medical Sciences not elsewhere specified
URN: urn:nbn:se:su:diva-74452OAI: diva2:509358
Available from: 2012-03-12 Created: 2012-03-12 Last updated: 2012-03-21Bibliographically approved
In thesis
1. Resin acids in commercial products and the work environment of Swedish wood pellets production: Analytical methodology, occurrence and exposure
Open this publication in new window or tab >>Resin acids in commercial products and the work environment of Swedish wood pellets production: Analytical methodology, occurrence and exposure
2012 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The aims of the work this thesis is based upon were to develop convenient analytical procedures for determining resin acids in biological and environmental matrices, and apply them to enhance understanding of the occurrence, exposure to and uptake by exposed individuals of resin acids. Particular focus has been on the workplace environment of the Swedish wood pellets industry. Sample extraction procedures and high-performance liquid chromatography/electrospray ionisation-mass spectrometry (HPLC/ESI-MS) methodologies were developed for measuring resin acids in dust, skin and urine samples. Chromatographic separation of abietic (AA) and pimaric acid was achieved by using a polar-embedded C12 stationary phase. The HPLC/ESI-MS method avoids undesirable oxidation of AA, which was found to occur during the derivatisation step in the standard MDHS 83/2 gas chromatography/flame ionisation detection (GC/FID) methodology, leading to false observations of both AA and the oxidation product 7-oxodehydroabietic acid (7-OXO). Personal exposures to resin acids in the Swedish wood pellet production industry were found to be lower, on average, than the British Occupational Exposure Limit for rosin (50 µg/m3). The oxidised resin acid 7-OXO, was detected in both dust and skin samples indicating the presence of allergenic resin acids. A correlation between air and post-shift urinary concentrations of dehydroabietic acid (DHAA), and a trend towards an increase in urinary 7-OXO during work shifts, were also observed. Whether the increase in 7-OXO was due to direct uptake or metabolism of other resin acids cannot be concluded from the results. An efficient HPLC/UV methodology with diode-array detection was developed for screening commercial products for rosin that could be used in laboratories lacking mass spectrometers. Very high concentrations of free resin acids were detected in depilatory wax strips using the method.

Place, publisher, year, edition, pages
Stockholm: Department of Analytical Chemistry, Stockholm University, 2012. 60 p.
Rosin, resin acids, HPLC/ESI-MS, air sampling, urine samples, wood pellets, wood dust
National Category
Analytical Chemistry
Research subject
Analytical Chemistry
urn:nbn:se:su:diva-74448 (URN)978-91-7447-449-7 (ISBN)
Public defence
2012-04-27, Magnélisalen, Kemiska övningslaboratoriet, Svante Arrhenius väg 16 B, Stockholm, 13:00 (English)
At the time of doctoral defense, the following paper was unpublished and had a status as follows: Paper 4: Submitted.Available from: 2012-04-03 Created: 2012-03-12 Last updated: 2012-03-28Bibliographically approved

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