Galanin receptor agonists protect against kainic acid-induced excitotoxicity in the rat hippocampus
2011 (English)Conference paper, Abstract (Refereed)
Galanin is a peptide neurotransmitter with neuroprotective actions. Administration of galanin or selective galanin receptor agonists to rats reduces convulsant-induced seizure behavior. However, it remains unclear whether galanin exerts a neuroprotective effect in vivo and which galanin receptor subtype may mediate this effect. The present experiments evaluated the impact of two separate galanin receptor agonists, M1145 and M1154, which are specific for GAL R2 and GAL R1/R2 respectively, on seizure behavior and neuronal excitotoxicity following intracerebroventricular (ICV) administration of kainic acid. Male, Sprague-Dawley rats received ICV infusions of M1145, M1154, or saline prior to an ICV infusion of kainic acid. Seizure behaviors were rated for 30 minutes post-infusion, and rats were euthanized and transcardially perfused 48 hours later. Twenty micron coronal sections of the hippocampal region were obtained and nissl-stained for microscopic cell counting of pyramidal neurons in the CA3 region.Neither galanin receptor agonist reduced the seizure behaviors induced by kainic acid. Cell counting in CA3 revealed that kainic acid treatment alone caused near complete destruction of cells. However, rats pretreated with the galanin receptor agonists showed reduced cell loss. These results provide further evidence of the protective role of galanin in the hippocampus and suggest that the GAL R2 receptor mediates these effects.
Place, publisher, year, edition, pages
2011. 39.26/D58- p.
Neuroprotection, CA3, Seizure, Galanin, Neuropetide, GalR1, GPCR
Biochemistry and Molecular Biology Neurosciences
Research subject Neurochemistry with Molecular Neurobiology
IdentifiersURN: urn:nbn:se:su:diva-75090OAI: oai:DiVA.org:su-75090DiVA: diva2:514070
Society for Neuroscience Annual Meeting, Washington, DC, USA, November 12-16, 2011