Crystal cell activation through rupture
(English)Manuscript (preprint) (Other academic)
Cell lysis is increasingly recognized as a mode of immune cell activation. We studied secretion by cell rupture using crystal cells, a specialized type of immune cell from the model insect
Drosophila melanogaster. Crystal cells contain a multifunctional enzyme in a proform within crystalline inclusions, which are released during rupture and dissolve within minutes. Using targeted screens we identified cytoskeletal components and regulators as mediators of cell rupture. We also find evidence for an involvement of the endocytic pathway and for caspases. In addition we find no evidence for a release of nuclear DNA. Taken together our results set crystal cell activation apart from other activation processes that involve cell lysis such as the release of neutrophil traps.
Research subject Molecular Biology
IdentifiersURN: urn:nbn:se:su:diva-75212OAI: oai:DiVA.org:su-75212DiVA: diva2:515019