Separation of Lipid Classes by HPLC on a Cyanopropyl Column
2012 (English)In: Lipids, ISSN 0024-4201, E-ISSN 1558-9307, Vol. 47, no 1, 93-99 p.Article in journal (Refereed) Published
A new method for the separation and identification of lipid classes by normal-phase HPLC on a cyanopropyl column is described. The use of a simple binary gradient, with toluene as a component, provided a rapid separation of non-polar as well as phospholipid classes. The inherent small differences in performances between possible non-polar eluent components of the gradient, such as hexane, heptane, and iso-octane, had a pronounced impact on retention times for individual phospholipid classes. Separation of molecular species within a lipid class could also be observed.
Place, publisher, year, edition, pages
2012. Vol. 47, no 1, 93-99 p.
Cyanopropyl column, Normal-phase HPLC, Lipid classes, Phospholipids, Toluene, Hexane, Heptane, Iso-octane
IdentifiersURN: urn:nbn:se:su:diva-76743DOI: 10.1007/s11745-011-3627-0ISI: 000299532400010OAI: oai:DiVA.org:su-76743DiVA: diva2:527211