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Structural studies of the O-antigenic polysaccharide from Plesiomonas shigelloides strain AM36565
Stockholm University, Faculty of Science, Department of Organic Chemistry.
Stockholm University, Faculty of Science, Department of Organic Chemistry.
Stockholm University, Faculty of Science, Department of Organic Chemistry.
Stockholm University, Faculty of Science, Department of Organic Chemistry.
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2012 (English)In: Carbohydrate Research, ISSN 0008-6215, E-ISSN 1873-426X, Vol. 348, 99-103 p.Article in journal (Refereed) Published
Abstract [en]

The structure of the repeating unit of the O-antigenic polysaccharide from Plesiomonas shigelloides strain AM36565 has been determined. Component analysis and H-1 and C-13 NMR spectroscopy experiments were employed to elucidate the structure. Inter-residue correlations were determined by H-1,C-13 heteronuclear multiple-bond correlation, H-1-H-1-NOESY, and H-1,C-13-HSQC-H-1,H-1-NOESY experiments. The O-antigen polysaccharide is composed of repeating units with the following structure: -> 3)-alpha-L-Rhap-(1 -> 2)-alpha-L-Rhap-(1 -> 4)[(beta-D-GalpNAc-(1 -> 3)]-alpha-D-GlcpNAc-(1 ->, in which the monosaccharide side-chain substitutes the backbone in half of the repeating units. A matrix-assisted laser desorption/ionization mass spectrometry experiment suggested that the polysaccharide consists of two regions, one with tetrasaccharide repeating units and one with trisaccharide repeating units.

Place, publisher, year, edition, pages
2012. Vol. 348, 99-103 p.
Keyword [en]
Plesiomonas shigelloides, Lipopolysaccharide, NMR, MALDI-TOF MS, SDS-PAGE, Biological repeating unit
National Category
Organic Chemistry
Identifiers
URN: urn:nbn:se:su:diva-78434DOI: 10.1016/j.carres.2011.10.021ISI: 000300926300014OAI: oai:DiVA.org:su-78434DiVA: diva2:539765
Funder
Swedish Research CouncilKnut and Alice Wallenberg Foundation
Note
7Available from: 2012-07-05 Created: 2012-07-04 Last updated: 2017-12-07Bibliographically approved

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