Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Cell-penetrating peptides and oligonucleotide delivery
Stockholm University, Faculty of Science, Department of Neurochemistry.ORCID iD: 0000-0002-1228-9927
2012 (English)Licentiate thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Stockholm: Department of Neurochemistry, Stockholm University , 2012. , 25 p.
National Category
Biological Sciences Chemical Sciences
Research subject
Neurochemistry with Molecular Neurobiology
Identifiers
URN: urn:nbn:se:su:diva-79060ISBN: 978-91-7447-530-2 (print)OAI: oai:DiVA.org:su-79060DiVA: diva2:546852
Opponent
Supervisors
Available from: 2012-08-28 Created: 2012-08-24 Last updated: 2015-03-16Bibliographically approved
List of papers
1. Modulating Anti-MicroRNA-21 Activity and Specificity Using Oligonucleotide Derivatives and Length Optimization
Open this publication in new window or tab >>Modulating Anti-MicroRNA-21 Activity and Specificity Using Oligonucleotide Derivatives and Length Optimization
Show others...
2012 (English)In: ISRN Pharmaceutics, ISSN 2090-6145, E-ISSN 2090-6153, Vol. 2012, 407154Article in journal (Refereed) Published
Abstract [en]

MicroRNAs are short, endogenous RNAs that direct posttranscriptional regulation of gene expression vital for many developmental and cellular functions. Implicated in the pathogenesis of several human diseases, this group of RNAs provides interesting targets for therapeutic intervention. Anti-microRNA oligonucleotides constitute a class of synthetic antisense oligonucleotides used to interfere with microRNAs. In this study, we investigate the effects of chemical modifications and truncations on activity and specificity of anti-microRNA oligonucleotides targeting microRNA-21. We observed an increased activity but reduced specificity when incorporating locked nucleic acid monomers, whereas the opposite was observed when introducing unlocked nucleic acid monomers. Our data suggest that phosphorothioate anti-microRNA oligonucleotides yield a greater activity than their phosphodiester counterparts and that a moderate truncation of the anti-microRNA oligonucleotide improves specificity without significantly losing activity. These results provide useful insights for design of anti-microRNA oligonucleotides to achieve both high activity as well as efficient mismatch discrimination.

National Category
Biological Sciences Chemical Sciences
Research subject
Neurochemistry with Molecular Neurobiology
Identifiers
urn:nbn:se:su:diva-79059 (URN)10.5402/2012/407154 (DOI)22474606 (PubMedID)
Funder
Knut and Alice Wallenberg FoundationSwedish Research Council
Available from: 2012-08-24 Created: 2012-08-24 Last updated: 2017-12-07Bibliographically approved
2. PepFect15, a novel endosomolytic cell-penetrating peptide for non-covalent oligonucleotide delivery
Open this publication in new window or tab >>PepFect15, a novel endosomolytic cell-penetrating peptide for non-covalent oligonucleotide delivery
Show others...
(English)Manuscript (preprint) (Other academic)
Keyword
cell-penetrating peptide, drug delivery, oligonucleotide
National Category
Chemical Sciences
Research subject
Neurochemistry with Molecular Neurobiology
Identifiers
urn:nbn:se:su:diva-74368 (URN)
Available from: 2012-03-08 Created: 2012-03-08 Last updated: 2016-01-29Bibliographically approved

Open Access in DiVA

No full text

Search in DiVA

By author/editor
Muñoz-Alarcón, Andrés
By organisation
Department of Neurochemistry
Biological SciencesChemical Sciences

Search outside of DiVA

GoogleGoogle Scholar

isbn
urn-nbn

Altmetric score

isbn
urn-nbn
Total: 49 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf