Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Neuroblastoma cells: in vitro studies on childhood cancer and amyloid precursor protein processing enzymes
Stockholm University, Faculty of Science, Department of Neurochemistry.
2009 (English)Licentiate thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Stockholm: Universitetsservice AB , 2009. , 54 p.
National Category
Natural Sciences
Research subject
Neurochemistry with Molecular Neurobiology
Identifiers
URN: urn:nbn:se:su:diva-79162ISBN: ISBN 978-91-7155-848-0 OAI: oai:DiVA.org:su-79162DiVA: diva2:547746
Presentation
2009-03-31, Heilbronnsalen, Svante Arrhenius väg 21A, 106 91 Stockholm, 16:50 (English)
Opponent
Supervisors
Available from: 2012-10-30 Created: 2012-08-28 Last updated: 2015-03-09Bibliographically approved
List of papers
1. Different strategies to inhibit NF-κB in order to sensitize Stypehuman neuroblastoma cells to TRAIL-induced apoptosis
Open this publication in new window or tab >>Different strategies to inhibit NF-κB in order to sensitize Stypehuman neuroblastoma cells to TRAIL-induced apoptosis
(English)Manuscript (preprint) (Other academic)
Keyword
Apoptosis, neuroblastoma, NF-κB, TRAIL
National Category
Biochemistry and Molecular Biology
Research subject
Neurochemistry with Molecular Neurobiology
Identifiers
urn:nbn:se:su:diva-79153 (URN)
Available from: 2012-08-28 Created: 2012-08-28 Last updated: 2016-01-29Bibliographically approved
2. PI3-K- and PKC-dependent up-regulation of APP processing enzymes by retinoic acid
Open this publication in new window or tab >>PI3-K- and PKC-dependent up-regulation of APP processing enzymes by retinoic acid
Show others...
2008 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 365, no 2, 298-303 p.Article in journal (Refereed) Published
Abstract [en]

Retinoic acid stimulates α-secretase processing of amyloid precursor protein (APP) and decreases β-secretase cleavage that leads to amyloid-β formation. Here, we investigated the effect of retinoic acid on the two putative α-secretases, the disintegrin metalloproteinases ADAM10 and TACE, and the β-site cleaving enzyme BACE1, in human neuroblastoma SH-SY5Y cells. Western blot analysis showed that exposure to retinoic acid resulted in significantly increased levels of ADAM10 and TACE, suggesting that regulation of α-secretases causes the effects on APP processing. The presence of the phosphatidylinositol 3-kinase inhibitor LY 294002 selectively reduced the effect on ADAM10 protein levels but not on ADAM10 mRNA levels as determined by RT-PCR. On the other hand, the effect on TACE was shown to be dependent on protein kinase C, since it was completely blocked in the presence of the inhibitor bisindolylmaleimide XI. Our data indicate that different signalling pathways are involved in retinoic acid-induced up-regulation of the secretases.

Keyword
ADAM10, APP, BACE1, BDNF, PI3-kinase, Protein kinase C, Retinoic acid, TACE
National Category
Natural Sciences
Identifiers
urn:nbn:se:su:diva-19985 (URN)10.1016/j.bbrc.2007.10.167 (DOI)000251494000015 ()
Available from: 2009-01-23 Created: 2009-01-23 Last updated: 2017-12-13Bibliographically approved

Open Access in DiVA

No full text

Search in DiVA

By author/editor
Gatsinzi, Tom
By organisation
Department of Neurochemistry
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar

isbn
urn-nbn

Altmetric score

isbn
urn-nbn
Total: 51 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf