Structural studies and biosynthetic aspects of the o antigen polysaccharide from Escherichia coli o174
2012 (English)In: Carbohydrate Research, ISSN 0008-6215, E-ISSN 1873-426X, Vol. 354, 102-105 p.Article in journal (Refereed) Published
The structure of the repeating unit of the O-antigenic polysaccharide (PS) from Escherichia coli O174 has been determined. Component analysis together with H-1 and C-13 NMR spectroscopy experiments were employed to elucidate the structure. Inter-residue correlations were determined by H-1, C-13-heteronuclear multiple-bond correlation and H-1, H-1-NOESY experiments. The PS is composed of tetrasaccharide repeating units with the following structure: -> 4)-beta-D-GlcpA-(1 -> 3)-beta-D-Galp-(1 -> 3)-beta-D-GalpNAc-(1 -> vertical bar beta-D-GlcpNAc-(1 -> 2) Cross-peaks of low intensity were present in the NMR spectra consistent with a beta-D-GlcpNAc-(1 -> 2)-beta-D-GlcpA(1 -> structural element at the terminal part of the polysaccharide, which on average is composed of similar to 15 repeating units. Consequently the biological repeating unit has a 3-substituted N-acetyl-D-galactosamine residue at its reducing end.
Place, publisher, year, edition, pages
2012. Vol. 354, 102-105 p.
Escherichia coli, Lipopolysaccharide, NMR, Biological repeating unit
Organic Chemistry Biochemistry and Molecular Biology
Research subject Organic Chemistry
IdentifiersURN: urn:nbn:se:su:diva-79797DOI: 10.1016/j.carres.2012.02.020ISI: 000304055100012OAI: oai:DiVA.org:su-79797DiVA: diva2:551561
FunderSwedish Research CouncilKnut and Alice Wallenberg Foundation