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A comparison of TO-PRO-1 iodide and 5-CFDA-AM staining methods for assessing viability of planktonic algae with epifluorescence microscopy
Stockholm University, Faculty of Science, Department of Applied Environmental Science (ITM). Stockholm University, Faculty of Science, Department of Systems Ecology.
Stockholm University, Faculty of Science, Department of Systems Ecology.
Stockholm University, Faculty of Science, Department of Systems Ecology.
2012 (English)In: Journal of Microbiological Methods, ISSN 0167-7012, E-ISSN 1872-8359, Vol. 89, no 3, 216-221 p.Article in journal (Refereed) Published
Abstract [en]

Two fluorescent dyes, TO-PRO-1 iodide and 5-CFDA-AM, were evaluated for LIVE/DEAD assessment of unicellular marine algae Brachiomonas submarina and Tetraselmis suecica. Epifluorescence microscopy was used to estimate cell viability in predetermined mixtures of viable and non-viable algal cells and validated using microplate growth assay as reference measurements. On average, 5-CFDA-AM underestimated live cell abundance by similar to 25% compared with viability estimated by the growth assay, whereas TO-PRO-1 iodide provided accurate viability estimates. Furthermore, viability estimates based on staining with TO-PRO-1 iodide were not affected by a storage period of up to one month in -80 degrees C, making the assay a good candidate for routine assessment of phytoplankton populations in field and laboratory studies.

Place, publisher, year, edition, pages
2012. Vol. 89, no 3, 216-221 p.
Keyword [en]
Algal cell viability, Carboxylluorescein diacetate acetoxymethyl ester, 5-CFDA-AM, LIVE/DEAD staining, Microplate growth assay, TO-PRO-1 iodide
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:su:diva-79883DOI: 10.1016/j.mimet.2012.03.005ISI: 000304506700012OAI: oai:DiVA.org:su-79883DiVA: diva2:551856
Note

AuthorCount:3;

Available from: 2012-09-12 Created: 2012-09-11 Last updated: 2017-12-07Bibliographically approved

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Gorokhova, ElenaSundström, Annica M.
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Department of Applied Environmental Science (ITM)Department of Systems Ecology
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