A comparison of TO-PRO-1 iodide and 5-CFDA-AM staining methods for assessing viability of planktonic algae with epifluorescence microscopy
2012 (English)In: Journal of Microbiological Methods, ISSN 0167-7012, E-ISSN 1872-8359, Vol. 89, no 3, 216-221 p.Article in journal (Refereed) Published
Two fluorescent dyes, TO-PRO-1 iodide and 5-CFDA-AM, were evaluated for LIVE/DEAD assessment of unicellular marine algae Brachiomonas submarina and Tetraselmis suecica. Epifluorescence microscopy was used to estimate cell viability in predetermined mixtures of viable and non-viable algal cells and validated using microplate growth assay as reference measurements. On average, 5-CFDA-AM underestimated live cell abundance by similar to 25% compared with viability estimated by the growth assay, whereas TO-PRO-1 iodide provided accurate viability estimates. Furthermore, viability estimates based on staining with TO-PRO-1 iodide were not affected by a storage period of up to one month in -80 degrees C, making the assay a good candidate for routine assessment of phytoplankton populations in field and laboratory studies.
Place, publisher, year, edition, pages
2012. Vol. 89, no 3, 216-221 p.
Algal cell viability, Carboxylluorescein diacetate acetoxymethyl ester, 5-CFDA-AM, LIVE/DEAD staining, Microplate growth assay, TO-PRO-1 iodide
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:su:diva-79883DOI: 10.1016/j.mimet.2012.03.005ISI: 000304506700012OAI: oai:DiVA.org:su-79883DiVA: diva2:551856