A search for reciprocal signaling between insulin and adipokinetic hormone producing cells in Drosophila
(English)Manuscript (preprint) (Other academic)
Insulin signaling in Drosophila regulates major physiological processes such as stress resistance, growth, carbohydrate and lipid metabolism, aging, reproduction and possibly diapause. Adipokinetic hormone (AKH) signaling originating from the corpora cardiaca (CC) cells, is another crucial regulator of hemolymph carbohydrate levels in Drosophila. These two systems are suggested to have opposing effects on lipid and sugar metabolism in Drosophila, reminiscent of the mammalian insulin and glucagon hormones. We studied the possible functional relationship between the insulin producing cells (IPCs) and the CC cells by interfering with insulin receptor (InR) and AKH receptor (AKH-R) expression on these cells. Our experiments revealed increased carbohydrate and lipid levels after knocking down InR in the CC cells. We also showed that diminished InR levels on the IPCs lead to increased starvation resistance, however we did not observe any changes in carbohydrate or lipid levels. So far we can only suggest action of insulins via its receptor on the IPCs and the CC cells, but we do not have conclusive data for AKH action on IPCs or CC cells.
Insulin signaling, insulin receptor, adipokinetic hormone receptor, metabolism, peptide hormones, Drosophila melanogaster
Research subject Functional Zoomorphology
IdentifiersURN: urn:nbn:se:su:diva-80322OAI: oai:DiVA.org:su-80322DiVA: diva2:552971
FunderSwedish Research Council