Change search
ReferencesLink to record
Permanent link

Direct link
Room temperature femtosecond X-ray diffraction of photosystem II microcrystals
Show others and affiliations
2012 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 109, no 25, 9721-9726 p.Article in journal (Refereed) Published
Abstract [en]

Most of the dioxygen on earth is generated by the oxidation of water by photosystem II (PS II) using light from the sun. This lightdriven, four-photon reaction is catalyzed by the Mn4CaO5 cluster located at the lumenal side of PS II. Various X-ray studies have been carried out at cryogenic temperatures to understand the intermediate steps involved in the water oxidation mechanism. However, the necessity for collecting data at room temperature, especially for studying the transient steps during the O-O bond formation, requires the development of new methodologies. In this paper we report room temperature X-ray diffraction data of PS II microcrystals obtained using ultrashort (<50 fs) 9 keV X-ray pulses from a hard X-ray free electron laser, namely the Linac Coherent Light Source. The results presented here demonstrate that the probe before destroy approach using an X-ray free electron laser works even for the highly-sensitive Mn4CaO5 cluster in PS II at room temperature. We show that these data are comparable to those obtained in synchrotron radiation studies as seen by the similarities in the overall structure of the helices, the protein subunits and the location of the various cofactors. This work is, therefore, an important step toward future studies for resolving the structure of the Mn4CaO5 cluster without any damage at room temperature, and of the reaction intermediates of PS II during O-O bond formation.

Place, publisher, year, edition, pages
2012. Vol. 109, no 25, 9721-9726 p.
Keyword [en]
manganese, oxygen-evolving complex
National Category
Physical Sciences
URN: urn:nbn:se:su:diva-80315DOI: 10.1073/pnas.1204598109ISI: 000306061400021OAI: diva2:553004


Available from: 2012-09-17 Created: 2012-09-17 Last updated: 2012-09-17Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Sellberg, Jonas
By organisation
Department of Physics
In the same journal
Proceedings of the National Academy of Sciences of the United States of America
Physical Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 23 hits
ReferencesLink to record
Permanent link

Direct link