In vivo versus in vitro individual radiosensitivity analysed in healthy donors and in prostate cancer patients with and without severe side effects after radiotherapy
2012 (English)In: International Journal of Radiation Biology, ISSN 0955-3002, E-ISSN 1362-3095, Vol. 88, no 5, 405-413 p.Article in journal (Refereed) Published
Background : A high cellular radiosensitivity may be connected with a risk for development of severe side effects after radiotherapy and indicate cancer susceptibility. Hence, a fast and robust in vitro test is desirable to identify radiosensitive individuals. Materials and methods : The study included 25 prostate cancer patients with severe side effects (S) and 25 patients without severe side effects (0) after radiotherapy as well as 23 male healthy age-matched donors. Blood samples were exposed to 0.5 Gy or 1 Gy of gamma-rays. The initial level of double-strand breaks (dsb) and repair kinetics measured by phosphorylation of histone H2A (gamma-H2AX-assay), apoptosis (Annexin V-assay) and the induction of chromatid aberrations after irradiation in the G2-phase of the cell cycle (G2-assay) were analysed. Results : A significant higher chromatid aberration yield was found in lymphocytes from prostate cancer patients when compared to healthy donors. We found no significant differences between patients S and patients 0. Conclusions : There is no obvious correlation between clinical and cellular radiosensitivity in lymphocytes of prostate cancer patients when all chosen in vitro assays are considered. Although 25% of the patients showed both severe side effects and increased radiation-induced chromosomal sensitivity, predictive value of G2-assay is doubtful.
Place, publisher, year, edition, pages
2012. Vol. 88, no 5, 405-413 p.
Individual radiosensitivity, chromatid aberrations, predictive assays, cancer susceptibility, DNA damage, prostate cancer
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:su:diva-80775DOI: 10.3109/09553002.2012.666002ISI: 000303495500003OAI: oai:DiVA.org:su-80775DiVA: diva2:557412