Change search
ReferencesLink to record
Permanent link

Direct link
Development of an HPLC Method for Determination of Related Impurities in Prilocaine Substance
Stockholm University, Faculty of Science, Department of Analytical Chemistry.
2012 (English)In: Chromatographia, ISSN 0009-5893, E-ISSN 1612-1112, Vol. 75, no 7-8, 329-336 p.Article in journal (Refereed) Published
Abstract [en]

Development of a reversed phase high performance liquid chromatographic method for determination of six related impurities in prilocaine substance is reported. The test of related impurities in European Pharmacopoeia (Ph. Eur.) cannot meet the demands with the chromatographic parameters given, therefore different types of chromatographic systems and eight columns have been evaluated in the present study. A new method with a Hypercarb column was developed and validated. This method fulfils the demands in the Ph. Eur., and the validation shows that the method is selective, reproducible, linear, accurate and robust with sufficient limits of detection (0.001-0.004% of 2.5 mg prilocaine mL(-1)) and quantification (0.002-0.009% of 2.5 mg prilocaine mL(-1)).

Place, publisher, year, edition, pages
2012. Vol. 75, no 7-8, 329-336 p.
Keyword [en]
LC, Prilocaine impurities, Local anaesthetics, Prilocaine, Hypercarb, Porous graphitized carbon
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:su:diva-80733DOI: 10.1007/s10337-012-2212-xISI: 000303349400002OAI: diva2:558702


Available from: 2012-10-04 Created: 2012-09-27 Last updated: 2012-10-04Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Granelli, Ingrid
By organisation
Department of Analytical Chemistry
In the same journal
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 28 hits
ReferencesLink to record
Permanent link

Direct link