Change search
ReferencesLink to record
Permanent link

Direct link
Chemosensory proteins, major salivary factors in caterpillar mandibular glands
Stockholm University, Faculty of Science, Department of Zoology, Animal Ecology.ORCID iD: 0000-0003-0296-0577
Stockholm University, Faculty of Science, Department of Zoology, Animal Ecology.
Stockholm University, Science for Life Laboratory (SciLifeLab).
Show others and affiliations
2012 (English)In: Insect Biochemistry and Molecular Biology, ISSN 0965-1748, E-ISSN 1879-0240, Vol. 42, no 10, 796-805 p.Article in journal (Refereed) Published
Abstract [en]

Research in the field of insect-host plant interactions has indicated that constituents of insect saliva play an important role in digestion and affect host chemical defense responses. However, most efforts have focused on studying the composition and function of regurgitant or saliva produced in the labial glands. Acknowledging the need for understanding the role of the mandibular glands in herbivory, we sought to make a qualitative and semi-quantitative comparison of soluble luminal fractions between mandibular and labial glands of Vanessa gonerilla butterfly larvae. Amylase and lysozyme were inspected as possible major enzymatic activities in the mandibular glands aiding in pre-digestion and antimicrobial defense. Although detected, neither of these enzymatic activities was prominent in the luminal protein preparation of a particular type of gland. Proteins isolated from the glands were identified by mass spectrometry and by searching an EST-library database generated for four other nymphalid butterfly species, in addition to the public NCBI database. The identified proteins were also quantified from thedata using “Quanty”, an in-house program. The proteomic analysis detected chemosensory proteins as the most abundant luminal proteins in the mandibular glands. In comparison to these proteins, the relative amounts of amylase and lysozyme were much lower in both gland types. Therefore, we speculate that the primary role of the mandibular glands in Lepidopteran larvae is chemoreception which may include the detection of microorganisms on plant surfaces, host plant recognition and communication with conspecifics.

Place, publisher, year, edition, pages
2012. Vol. 42, no 10, 796-805 p.
Keyword [en]
Mandibular glands, Chemosensory protein, Lysozyme, Amylase, Caterpillar, Saliva, Phyllosphere, Insect–host plant interaction, Proteomics, Mass spectrometry
National Category
Biochemistry and Molecular Biology
Research subject
Zoology; Molecular Biology
URN: urn:nbn:se:su:diva-81231DOI: 10.1016/j.ibmb.2012.07.008ISI: 000309620400008OAI: diva2:560526
Available from: 2012-10-15 Created: 2012-10-15 Last updated: 2014-10-28Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Celorio-Mancera, Maria de la PazJanz, Niklas
By organisation
Animal EcologyScience for Life Laboratory (SciLifeLab)
In the same journal
Insect Biochemistry and Molecular Biology
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 325 hits
ReferencesLink to record
Permanent link

Direct link