Spatio temporal regulation of adar editing during development in porcine neural tissues
2012 (English)In: RNA Biology, ISSN 1547-6286, Vol. 9, no 8, 1054-1065 p.Article in journal (Refereed) Published
Editing by ADAR enzymes is essential for mammalian life. Still, knowledge of the spatio-temporal editing patterns in mammals is limited. By use of 454 amplicon sequencing we examined the editing status of 12 regionally extracted mRNAs from porcine developing brain encompassing a total of 64 ADAR editing sites. In total 24 brain tissues, dissected from up to five regions from embryonic gestation day 23, 42, 60, 80, 100 and 115, were examined for editing. Generally, editing increased during embryonic development concomitantly with an increase in ADAR2 mRNA level. Notably, the Gria2 (GluR-B) Q/R site, reported to be similar to 100% edited in previous studies, is only 54% edited at embryonic day 23. Transcripts with multiple editing sites in close proximity to each other exhibit coupled editing and an extraordinary incidence of long-range coupling of editing events more than 32 kb apart is observed for the kainate glutamate receptor 2 transcript, Grik2. Our study reveals complex spatio-temporal ADAR editing patterns of coordinated editing events that may play important roles in the development of the mammalian brain.
Place, publisher, year, edition, pages
2012. Vol. 9, no 8, 1054-1065 p.
ADAR2, ADAR, Blcap, Pig, Htr2c, 5-HT2C receptor, RNA editing, Gria2, GluR-B
IdentifiersURN: urn:nbn:se:su:diva-81734DOI: 10.4161/rna.21082ISI: 000308590400004OAI: oai:DiVA.org:su-81734DiVA: diva2:563559