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Nanoflow electrospinning serial femtosecond crystallography
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2012 (English)In: Acta Crystallographica Section D: Biological Crystallography, ISSN 0907-4449, E-ISSN 1399-0047, Vol. 68, 1584-1587 p.Article in journal (Refereed) Published
Abstract [en]

An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14-3.1 mu l min(-1) to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 mu l min(-1) and diffracted to beyond 4 angstrom resolution, producing 14 000 indexable diffraction patterns, or four per second, from 140 mu g of protein. Nanoflow electrospinning extends SFX to biological samples that necessitate minimal sample consumption.

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2012. Vol. 68, 1584-1587 p.
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Biochemistry and Molecular Biology Biophysics
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URN: urn:nbn:se:su:diva-83025DOI: 10.1107/S0907444912038152ISI: 000310069500017OAI: oai:DiVA.org:su-83025DiVA: diva2:574421
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AuthorCount:38;

Available from: 2012-12-05 Created: 2012-12-03 Last updated: 2017-12-07Bibliographically approved

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