Quantitative analysis of SecYEG-mediated insertion of transmembrane α-helices into the bacterial inner membrane
2012 (English)Manuscript (preprint) (Other academic)
Most integral membrane proteins, both inprokaryotic and eukaryotic cells, are cotranslationallyinserted into the membrane viaSec-type translocons: the SecYEG complex inprokaryotes and the Sec61 complex ineukaryotes. The contributions of individualamino acids to the overall free energy ofmembrane insertion of single transmembraneα-helices have been measured for Sec61-mediated insertion into the endoplasmicreticular (ER) membrane (Nature 450:1026-1030), but have not been systematicallydetermined for SecYEG-mediated insertioninto the bacterial inner membrane. We nowreport such measurements, carried out inEscherichia coli. Overall, there is a goodcorrelation between the results found for themammalian ER and the E. coli innermembrane, but the hydrophobicity thresholdfor SecYEG-mediated insertion is distinctlylower than for Sec61-mediated insertion.
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IdentifiersURN: urn:nbn:se:su:diva-83482OAI: oai:DiVA.org:su-83482DiVA: diva2:575965