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Transient small molecule interactions kinetically modulate amyloid beta peptide self-assembly
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
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2012 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 586, no 22, 3991-3995 p.Article in journal (Refereed) Published
Abstract [en]

Small organic molecules, like Congo red and lacmoid, have been shown to modulate the self-assembly of the amyloid beta peptide (A beta). Here, we show that A beta forms NMR invisible non-toxic co-aggregates together with lacmoid as well as Congo red. We find that the interaction involves two distinct kinetic processes and at every given time point only a small fraction of A beta is in the co-aggregate. These weak transient interactions kinetically redirect the aggregation prone A beta from self-assembling into amyloid fibrils. These findings suggest that even such weak binders might be effective as therapeutics against pathogenic protein aggregation.

Place, publisher, year, edition, pages
2012. Vol. 586, no 22, 3991-3995 p.
Keyword [en]
Amyloid, Alzheimer's disease, NMR relaxation dispersion, Dynamic exchange
National Category
Biochemistry and Molecular Biology
Research subject
Biophysics
Identifiers
URN: urn:nbn:se:su:diva-83815DOI: 10.1016/j.febslet.2012.09.035ISI: 000310783800010OAI: oai:DiVA.org:su-83815DiVA: diva2:577121
Note

AuthorCount:5;

Available from: 2012-12-14 Created: 2012-12-14 Last updated: 2017-12-06Bibliographically approved
In thesis
1. Modulation of amyloid β peptide self-assembly: Aggregation mechanisms associated with Alzheimer's disease
Open this publication in new window or tab >>Modulation of amyloid β peptide self-assembly: Aggregation mechanisms associated with Alzheimer's disease
2013 (English)Licentiate thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Stockholm: Department of Biochemistry and Biophysics, Stockholm University, 2013. 22 p.
National Category
Biophysics
Research subject
Biophysics
Identifiers
urn:nbn:se:su:diva-89078 (URN)
Presentation
2013-04-09, Magnéli hall, 14:30 (English)
Opponent
Supervisors
Available from: 2013-04-10 Created: 2013-04-10 Last updated: 2015-02-23Bibliographically approved
2. Modulation of Alzheimer's amyloid β peptide self-assembly: Insights into molecular mechanisms of peptide aggregation associated with Alzheimer's disease
Open this publication in new window or tab >>Modulation of Alzheimer's amyloid β peptide self-assembly: Insights into molecular mechanisms of peptide aggregation associated with Alzheimer's disease
2015 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Misfolding of proteins and peptides is closely linked to several neurodegenerative disorders, among them Alzheimer's disease (AD), the most prominent example of brain diseases. The self-assembly of the amyloid β peptide (Aβ) into amyloid fibrils is one histologic hallmark of AD. A detailed knowledge about the underlying mechanism(s) of Aβ aggregation is crucial for advances toward a fundamental understanding of the disease, which may promote the search for and design of efficient therapeutics. The work presented in this thesis deals with modulation of the aggregation process by various compounds, i.e. small organic molecules (e.g. lacmoid and Congo red), surfactants and metal ions. These results provide insight into the molecular mechanism of modulator interactions and interference with Aβ and its aggregation pathways. Applying a combination of kinetic and dynamic studies as well as structural investigations we characterized the molecular interactions between Aβ and aggregation modulators in terms of microscopic rate constants, conformational preferences and thermodynamics. An important conclusion is that these modulators form highly dynamic complexes with Aβ, with life-times on the timescale of milliseconds. Despite the similar exchange dynamics, the effect on peptide aggregation is modulator-specific and fibril formation can be accelerated, retarded or inhibited by their interactions. In summary, Aβ self-assembly is governed by microscopic kinetic and dynamic processes that can be altered by aggregation modulators. Further elucidation of these mechanisms is beneficial for the understanding and therapeutic intervention of amyloid diseases.

Place, publisher, year, edition, pages
Stockholm: Department of Biochemistry and Biophysics, Stockholm University, 2015
National Category
Biophysics
Research subject
Biophysics
Identifiers
urn:nbn:se:su:diva-114172 (URN)978-91-7649-104-1 (ISBN)
Public defence
2015-03-27, Magnéli hall, Arrhenius Laboratory, Svante Arrhenius väg 16 B, Stockholm, 10:00 (English)
Opponent
Supervisors
Note

At the time of the doctoral defense, the following paper was unpublished and had a status as follows: Paper 4: Manuscript.

Available from: 2015-03-05 Created: 2015-02-23 Last updated: 2015-04-09Bibliographically approved

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