N-Acyl taurines trigger insulin secretion by increasing calcium flux in pancreatic beta-cells
2013 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 430, no 1, 54-59 p.Article in journal (Refereed) Published
Pancreatic beta-cells secrete insulin in response to various stimuli to control blood glucose levels. This insulin release is the result of a complex interplay between signaling, membrane potential and intracellular calcium levels. Various nutritional and hormonal factors are involved in regulating this process. N-Acyl taurines are a group of fatty acids which are amidated (or conjugated) to taurine and little is known about their physiological functions. In this study, treatment of pancreatic beta-cell lines (HIT-T15) and rat islet cell lines (INS-1) with N-acyl taurines (N-arachidonoyl taurine and N-oleoyl taurine), induced a high frequency of calcium oscillations in these cells. Treatment with N-arachidonoyl taurine and N-oleoyl taurine also resulted in a significant increase in insulin secretion from pancreatic beta-cell lines as determined by insulin release assay and immunofluorescence (p < 0.05). Our data also show that the transient receptor potential vanilloid 1 (TRPV1) channel is involved in insulin secretion in response to N-arachidonoyl taurine and N-oleoyl taurine treatment. However our data also suggest that receptors other than TRPV1 are involved in the insulin secretion response to treatment with N-oleoyl taurine.
Place, publisher, year, edition, pages
2013. Vol. 430, no 1, 54-59 p.
N-Arachidonoyl taurine, N-Oleoyl taurine, Insulin secretion, Transient receptor potential vanilloid, receptor 1 (TRPV1), Pancreatic beta-cells
IdentifiersURN: urn:nbn:se:su:diva-88357DOI: 10.1016/j.bbrc.2012.11.026ISI: 000314320700010OAI: oai:DiVA.org:su-88357DiVA: diva2:613018