Change search
ReferencesLink to record
Permanent link

Direct link
Characterization of recombinant antibodies for cancer therapy by infrared spectroscopy
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
Stockholm University, Faculty of Science, Department of Biochemistry and Biophysics.
2013 (English)In: Biologicals (Print), ISSN 1045-1056, E-ISSN 1095-8320, Vol. 41, no 2, 104-110 p.Article in journal (Refereed) Published
Abstract [en]

Fourier transform infrared (FTIR) spectroscopy was used to study the structure of the recombinant antibodies 1E10, anti-CD20 and hR3, which are used as anti-cancer therapeutic drugs. We tested their sensitivity against different conditions and treatments such as pH, temperature, freeze-thaw cycles and drying, which are relevant for the practical usefulness of the drugs. All antibodies were stable against moderate temperature increases (up to 50 degrees C) and pH changes (range 5-9). 1E10 was sensitive to extreme pH values (pH 3 and 12), whereas hR3 was most sensitive to temperature (at and above 60 degrees C). We did not observe any significant changes upon freeze-thaw and drying treatments. The secondary structure content of all three antibodies was estimated to be similar to that of IgG with similar to 64% beta-sheet, 0% alpha-helix and similar to 36% other structure. (C) 2012 The International Alliance for Biological Standardization.

Place, publisher, year, edition, pages
2013. Vol. 41, no 2, 104-110 p.
Keyword [en]
FTIR spectroscopy, IR spectroscopy, Cancer, Anti-cancer drug, Recombinant antibody
National Category
Biochemistry and Molecular Biology Pharmacology and Toxicology
URN: urn:nbn:se:su:diva-89725DOI: 10.1016/j.biologicals.2012.11.004ISI: 000316843700008OAI: diva2:620206
Knut and Alice Wallenberg Foundation


Available from: 2013-05-08 Created: 2013-05-06 Last updated: 2013-05-08Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Barth, Andreas
By organisation
Department of Biochemistry and Biophysics
In the same journal
Biologicals (Print)
Biochemistry and Molecular BiologyPharmacology and Toxicology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 67 hits
ReferencesLink to record
Permanent link

Direct link